Portal de Periódicos da CAPES

Intracellular and extracellular spaces and the direct quantification of molar intracellular concentrations of phosphorus metabolites in the isolated rat heart using 31 P NMR spectroscopy and phosphonate markers

1994; Wiley; Volume: 32; Issue: 2 Linguagem: Inglês

10.1002/mrm.1910320206

1522-2594

Kieran Clarke, R. E. Anderson, Jean‐François Nédélec, David O. Foster, A.I. Ally,

Electron Spin Resonance Studies

Abstract To quantify metabolite and cation concentrations using NMR spectroscopy, the volumes of intracellular and extracellular spaces must be known. We describe a simple 31 P NMR spectroscopic method that employs dimethyl methylphosphonate (DMMP) as a marker of total water space and phenylphosphoriate (PPA) as a marker of extracellular space to determine intracellular and extracellular space volumes in the isolated, perfused rat heart. In vivo and in vitro radiolabel studies were used to verify this method. The difference between the total and extracellular water spaces, determined as milliliters/heart, gave the intracellular volume and allowed direct calculation of myocardial creatine phosphate, ATP, and inorganic phosphate concentrations, which were 13.4 m M , 10.1 m M , and 3.4 m M , respectively, for the glucose‐perfused rat heart. The extracellular volume decreased by 84% in hearts subjected to 28 min total, global ischemia and increased by 15% during reperfusion. The method described allows the determination of intracellular energy metabolite concentrations in perfused rat heart directly from a single, fully relaxed 31 P NMR spectrum.