Produção Nacional
Revisado por pares
In Vivo Bioinsecticidal Activity toward Ceratitis capitata (Fruit Fly) and Callosobruchus maculatus (Cowpea Weevil) and in Vitro Bioinsecticidal Activity toward Different Orders of Insect Pests of a Trypsin Inhibitor Purified from Tamarind Tree ( Tamarindus indica ) Seeds
2005; American Chemical Society; Volume: 53; Issue: 11 Linguagem: Inglês
10.1021/jf0502505
ISSN1520-5118
AutoresCarina L. Araújo, Ingrid Wilza Leal Bezerra, Adeliana S. Oliveira, Fabiano Tavares de Moura, Leonardo Lima Pepino Macedo, Carlos Eduardo Maia Gomes, Aulus Estevão Anjos de Deus Barbosa, Francisco P. Macedo, Tánia M. S. Souza, Octávio Luiz Franco, Carlos Bloch, Maurı́cio P. Sales,
Tópico(s)Agricultural pest management studies
ResumoA proteinaceous inhibitor with high activity against trypsin-like serine proteinases was purified from seeds of the tamarind tree (Tamarindus indica) by gel filtration on Shephacryl S-200 followed by a reverse-phase HPLC Vidac C18 TP. The inhibitor, called the tamarind trypsin inhibitor (TTI), showed a Mr of 21.42 kDa by mass spectrometry analysis. TTI was a noncompetitive inhibitor with a Ki value of 1.7 × 10-9 M. In vitro bioinsecticidal activity against insect digestive enzymes from different orders showed that TTI had remarkable activity against enzymes from coleopteran, Anthonomus grandis (29.6%), Zabrotes subfasciatus (51.6%), Callosobruchus maculatus (86.7%), Rhyzopertha dominica (88.2%), and lepidopteron, Plodia interpuncptella (26.7%), Alabama argillacea (53.8%), and Spodoptera frugiperda (75.5%). Also, digestive enzymes from Diptera, Ceratitis capitata (fruit fly), were inhibited (52.9%). In vivo bioinsecticidal assays toward C. capitata and C. maculatus larvae were developed. The concentration of TTI (w/w) in the artificial seed necessary to cause 50% mortality (LD50) of larvae was 3.6%, and that to reduce mass larvae by 50.0% (ED50) was 3.2%. Furthermore, the mass C. capitata larvae were affected at 53.2% and produced ∼34% mortality at a level of 4.0% (w/w) of TTI incorporated in artificial diets. Keywords: Tamarindus indica; trypsin inhibitor; bioinsecticide activity; insect pests
Referência(s)