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Fourier transform infrared imaging as a method for detection of HLA class I expression in melanoma without the use of antibody

2007; Wiley; Volume: 69; Issue: s1 Linguagem: Inglês

10.1111/j.1399-0039.2006.00775.x

1399-0039

Shit F. Chew, Bayden R. Wood, Carmel Kanaan, Jamie A. Browning, Duncan MacGregor, I. D. Davis, Jonathan Cebon, Brian D. Tait, D. McNaughton,

Animal Virus Infections Studies

Human leukocyte antigen (HLA) class I expression in melanoma is usually assessed using immunohistochemical staining. Here we report on the use of Fourier transform infrared (FTIR) hyperspectral imaging, a method widely used in two-dimensional analysis of chemical components, to study HLA class I expression in tissue. Two-dimensional cluster colour images derived from unsupervised hierarchical cluster analysis of FTIR hyperspectral data on melanoma sections were compared with consecutive sections that were immunohistochemically stained for class I expression. HLA-class-I-positive and -negative areas were differentiated by FTIR cluster images in all eight melanoma sections investigated without the need for antibody attachment. FTIR imaging enables the distinction of HLA-class-I-positive from class-I-negative areas in melanoma. This method is accurate, rapid and cost-effective.