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Antileishmanial activity of indole alkaloids from Aspidosperma ramiflorum

2006; Elsevier BV; Volume: 14; Issue: 6 Linguagem: Inglês

10.1016/j.phymed.2006.09.002

1618-095X

J.C.A. Tanaka, C.C. da Silva, Izabel Cristina Piloto Ferreira, Gérzia M. C. Machado, Leonor L. Leon, Arildo José Braz de Oliveira,

Plant Toxicity and Pharmacological Properties

The present study was designated to evaluate the antileishmanial activity of acid and basic fractions that were obtained after acid-basic extraction, from ethanolic 70% crude extract and pure compounds from the stem bark of Aspidosperma ramiflorum. The basic alkaloidal fraction presented a good activity against the extracellular form (promastigotes) of Leishmania (L.) amazonensis (LD50 value<47 μg/ml). Based on these findings, the basic fraction was fractionated on silica gel column chromatography in a bioassay-guided fractionation affording individual purified ramiflorines A and B. Both ramiflorines A and B showed significant activity against Leishmania (L.) amazonensis (LD50 values of 16.3±1.6 μg/ml and 4.9±0.9 μg/ml, respectively). Our results are promising, showing that these compounds are biologically active against Gram-positive bacteria.