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Identification of glycine spin systems in 1 H NMR spectra of proteins using multiple quantum coherences

1985; Wiley; Volume: 186; Issue: 1 Linguagem: Inglês

10.1016/0014-5793(85)81334-x

1873-3468

Jonathan Boyd, Christopher M. Dobson, Christina Redfield,

Molecular spectroscopy and chirality

Double‐quantum filtered COSY and triple‐quantum filtered COSY techniques have been compared for the tripeptide Gly‐Tyr‐Gly and for human lysozyme. The insertion of a triple‐quantum filter in the COSY experiment leads to dramatic spectral simplification in the fingerprint region of the spectrum and permits the specific identification of glycine spin systems in the complex 1 H NMR spectra of proteins. The assignment of these peaks to glycine H α can be confirmed using 2D double‐quantum correlated spectroscopy.