High-performance liquid chromatographic determination of the enantiomers of carnitine and acetylcarnitine on a chiral stationary phase
1994; Elsevier BV; Volume: 673; Issue: 1 Linguagem: Inglês
10.1016/0021-9673(94)87055-1
ISSN1873-3778
AutoresTakahiro Hirota, K. Minato, Kazuhiro Ishii, Noriyuki Nishimura, Tadashi Sato,
Tópico(s)Metabolomics and Mass Spectrometry Studies
ResumoA method was developed for determining the respective d-isomers in l-carnitine and acetyl-l-carnitine by high-performance liquid chromatography (HPLC). dl-Carnitine and acetyl-dl-carnitine were derivatized with 9-anthryldiazomethane to increase the interaction between the solutes and a chiral stationary phase and also the sensitivity; the simple derivatization was carried out under mild conditions of standing at 50°C for 20 min. The enantiomeric separations of the respective derivatives were achieved by HPLC on a commercially available chiral column (Chiralcel OD-R) with a mobile phase of 0.5 M sodium perchlorate solution-acetonitrile (3:2). It was confirmed that this method shows good specificity, reproducibility, linearity, accuracy and sensitivity (detection limit for acetyl-d-carnitine ≈0.01%).
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