Real-Time Detection of Hyperosmotic Stress Response in Optically Trapped Single Yeast Cells Using Raman Microspectroscopy
2005; American Chemical Society; Volume: 77; Issue: 8 Linguagem: Inglês
10.1021/ac048359j
ISSN1520-6882
AutoresGajendra Singh, Caitriona M. Creely, Giovanni Volpe, Helga Grötsch, Dmitri Petrov,
Tópico(s)Bee Products Chemical Analysis
ResumoLiving cells survive environmentally stressful conditions by initiating a stress response. We monitored changes in the Raman spectra of optically trapped Saccharomyces cerevisiae yeast cell under normal, heat-treated, and hyperosmotic stress conditions. It is shown that when glucose was used to exert hyperosmotic stress, two chemical substancesglycerol and ethanolcan be monitored in real time in a single cell.
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