Determination of 5α-androstane-3α, 17β-diol and 5α-androstane-3β, 17β-diol in human plasma by selected ion monitoring
1986; Wiley; Volume: 13; Issue: 8 Linguagem: Inglês
10.1002/bms.1200130802
ISSN2376-3876
AutoresF. Jacolot, D. Picart, F. Berthou, H.H. Floch,
Tópico(s)Mass Spectrometry Techniques and Applications
Resumo5α-Androstane-3α, 17β-diol (3α-diol) and 5α-androstane-3β, 17β-diol (3β-diol) were measured in human peripheral plasma by gas chromatography-selected ion monitoring as their t-butyl dimethylsilyl ethers. The purification of the plasma extract involved a Sep-Pak® C18 cartridge and high-performance liquid chromatography. The concentrations (mean ± SD) of the two diols in four normal males are: 213 ± 111 pg/ml for the 3α-diol and 246 ± 133 pg/ml for the 3β-diol.
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