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Dense-Gas Chromatography of Nonvolatile Substances of High Molecular Weight

1968; American Association for the Advancement of Science; Volume: 159; Issue: 3811 Linguagem: Inglês

10.1126/science.159.3811.197

1095-9203

Lilian McLaren, Marcus N. Myers, J. Calvin Giddings,

Catalysis and Oxidation Reactions

Working at pressures of up to 2000 atmospheres, more than ten times higher than in previous gas chromatography, we used the solvent power of dense gases to enable migration of chromatographic substances of molecular weights as high as 400,000. Carotenoids, corticol steroids, sterols, nucleosides, amino acids, carbohydrates, and several polymers have been caused to migrate, separated, and detected in NH 3 and CO 2 carrier gases at temperatures of 140° and 40°C, just above the respective critical points. Previously such compounds either defied separation by gas chromatography or had to be chromatographed as their more volatile derivatives.