Sensing of Gram-positive bacteria in Drosophila: GNBP1 is needed to process and present peptidoglycan to PGRP-SA
2006; Springer Nature; Volume: 25; Issue: 20 Linguagem: Inglês
10.1038/sj.emboj.7601363
ISSN1460-2075
AutoresLihui Wang, Alexander N.R. Weber, Magda L. Atilano, Sérgio R. Filipe, Nicholas J Gay, Petros Ligoxygakis,
Tópico(s)Insect Resistance and Genetics
ResumoArticle5 October 2006free access Sensing of Gram-positive bacteria in Drosophila: GNBP1 is needed to process and present peptidoglycan to PGRP-SA Lihui Wang Lihui Wang Genetics Unit, Department of Biochemistry, University of Oxford, Oxford, UK Search for more papers by this author Alexander NR Weber Alexander NR Weber Department of Biochemistry, University of Cambridge, Cambridge, UKPresent address: German Cancer Research Centre, Im Neuenheimer Feld, 69120 Heidelberg, Germany Search for more papers by this author Magda L Atilano Magda L Atilano Instituto de Tecnologia Química e Biológica/Universidade Nova de Lisboa, Av. da República (EAN), Oeiras, Portugal Search for more papers by this author Sergio R Filipe Sergio R Filipe Instituto de Tecnologia Química e Biológica/Universidade Nova de Lisboa, Av. da República (EAN), Oeiras, Portugal Search for more papers by this author Nicholas J Gay Nicholas J Gay Department of Biochemistry, University of Cambridge, Cambridge, UK Search for more papers by this author Petros Ligoxygakis Corresponding Author Petros Ligoxygakis Genetics Unit, Department of Biochemistry, University of Oxford, Oxford, UK Search for more papers by this author Lihui Wang Lihui Wang Genetics Unit, Department of Biochemistry, University of Oxford, Oxford, UK Search for more papers by this author Alexander NR Weber Alexander NR Weber Department of Biochemistry, University of Cambridge, Cambridge, UKPresent address: German Cancer Research Centre, Im Neuenheimer Feld, 69120 Heidelberg, Germany Search for more papers by this author Magda L Atilano Magda L Atilano Instituto de Tecnologia Química e Biológica/Universidade Nova de Lisboa, Av. da República (EAN), Oeiras, Portugal Search for more papers by this author Sergio R Filipe Sergio R Filipe Instituto de Tecnologia Química e Biológica/Universidade Nova de Lisboa, Av. da República (EAN), Oeiras, Portugal Search for more papers by this author Nicholas J Gay Nicholas J Gay Department of Biochemistry, University of Cambridge, Cambridge, UK Search for more papers by this author Petros Ligoxygakis Corresponding Author Petros Ligoxygakis Genetics Unit, Department of Biochemistry, University of Oxford, Oxford, UK Search for more papers by this author Author Information Lihui Wang1, Alexander NR Weber2, Magda L Atilano3, Sergio R Filipe3, Nicholas J Gay2 and Petros Ligoxygakis 1 1Genetics Unit, Department of Biochemistry, University of Oxford, Oxford, UK 2Department of Biochemistry, University of Cambridge, Cambridge, UK 3Instituto de Tecnologia Química e Biológica/Universidade Nova de Lisboa, Av. da República (EAN), Oeiras, Portugal *Corresponding author. Genetics Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, Oxfordshire OX1 3QU, UK. Tel.: +44 1865 275314; Fax: +44 1865 275318; E-mail: [email protected] The EMBO Journal (2006)25:5005-5014https://doi.org/10.1038/sj.emboj.7601363 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Genetic evidence indicates that Drosophila defense against Gram-positive bacteria is mediated by two putative pattern recognition receptors acting upstream of Toll, namely Gram-negative binding protein 1 (GNBP1) and peptidoglycan recognition protein SA (PGRP-SA). Until now however, the molecular recognition proceedings for sensing of Gram-positive pathogens were not known. In the present, we report the physical interaction between GNBP1 and PGRP-SA using recombinant proteins. GNBP1 was able to hydrolyze Gram-positive peptidoglycan (PG), while PGRP-SA bound highly purified PG fragments (muropeptides). Interaction between these proteins was enhanced in the presence of PG or muropeptides. PGRP-SA binding depended on the polymerization status of the muropeptides, pointing to constraints in the number of PGRP-SA molecules bound for signaling initiation. We propose a model whereby GNBP1 presents a processed form of PG for sensing by PGRP-SA and that a tripartite interaction between these proteins and PG is essential for downstream signaling. Introduction The ability to detect pathogens is an essential component of the immune system. The first line of sensing is stimulated by the recognition of conserved components of microorganisms called pathogen-associated molecular patterns (PAMPs; Janeway and Medzhitov, 2002). These PAMPs consist of molecules not found in the host, including bacterial cell wall peptidoglycan (PG) and lipopolysaccharide. Recognition of PAMPs by specific proteins called pattern recognition receptors (PRRs) activates a broad specificity inflammatory response characterized by the recruitment and activation of phagocytes and the production of antimicrobial compounds. These types of innate defenses are well conserved from invertebrates to mammals (Hoffmann et al, 1999). Some of the most prevalent and severe infections known to medicine are caused by Gram-positive bacteria, the cell wall of which presents multiple layers of two insoluble, networked carbohydrates, namely, teichoic acid and PG (Sriskandan and Cohen, 1999; Brown, 2004). The latter is composed of β-1,4 carbohydrate chains, alternating N-acetylglucosamine and N-acetyl muramic acid sugars crosslinked by short peptide chains called stem peptides (Weber et al, 2003b). The third residue of the stem peptide is commonly a lysine (Lys-type PG) although some Bacilli contain diaminopimelic acid (DAP), a residue usually found in Gram-negative PG (Weber et al, 2003b). There is considerable variation in the modes of crosslinking between stem peptides as well as the residues included in the inter-peptide bridges. This heterogeneity in even the basic cell wall composition has confounded efforts to fit the Gram-positive innate immune response to the elegant concept of pattern recognition. It is thought that recognition of these pathogens is accomplished by several different strategies, some of which are among the most ancient and conserved elements of the innate immune response (Weber et al, 2003b). To address the question of PG recognition by the host, we have used Drosophila as a genetically tractable model, which relies solely on innate defenses to fight off infection. These defenses are centered around two pathways, the Toll signaling pathway, which is triggered following fungal or Gram-positive bacterial infection and the IMD (for immune deficiency) signaling pathway activated in Gram-negative sepsis. These cascades culminate in NF-κB-dependent regulation of many target genes including those coding for potent antimicrobial peptides (AMPs; reviewed in Leclerc and Reichhart, 2004; Wang and Ligoxygakis, 2006). Genetic screens have identified two putative PRRs as necessary for activating Toll following Gram-positive bacterial infection. PG recognition protein SA (PGRP-SA) and Gram-negative binding protein 1 (GNBP1) are blood-circulating proteins that were shown to be essential components of signaling upstream of Toll (Michel et al, 2001; Gobert et al, 2003). Point mutations in PGRP-SA and RNAi-mediated knock down of GNPB1 or deletions of its open reading frame led to severe defects in AMP expression and rendered defective flies susceptible to infection (Michel et al, 2001; Gobert et al, 2003; Pili-Floury et al, 2004). Concomitant overexpression of both proteins resulted in challenge-independent AMP activation (Gobert et al, 2003). In addition, structural studies of PGRP-SA have identified a binding domain for muropeptides and a protein–protein interaction surface that could serve as a platform for a potential interface with GNBP1 (Chang et al, 2004; Reiser et al, 2004). Finally, two recent papers describing the complex of the human PGRP-Iα with a muramyl peptapeptide (MPP) from Gram-positive bacteria have highlighted the induced structural rearrangements in the PG-binding domain that are essential for entry of the MPP and at the same time the way in which PGRPs could discriminate between Lys and DAP types of PG (Guan et al, 2006; Swaminathan et al, 2006). We have previously determined that injection of intact Lys-PG in PGRP-SA- or GNBP1-deficient flies does not result in Toll activation (Filipe et al, 2005). In contrast, injections of highly purified fragments of Gram-positive Lys-PG were able to trigger the Toll pathway in a GNBP1 but not in a PGRP-SA mutant background (Filipe et al, 2005). In addition, hemolymph (the insect analog of the mammalian blood) from wild-type flies had a hydrolyzing activity against Lys-PG, which was missing from GNBP1 mutant blood (Filipe et al, 2005). Taken together, these data formulated the hypothesis that GNBP1 could present Lys-PG to PGRP-SA while an interaction involving the two proteins and PG could be envisaged (Filipe et al, 2005). To clarify the molecular recognition mechanism of Gram-positive bacteria by the Drosophila host, we produced recombinant GNBP1 and PGRP-SA in a baculovirus expression system. Here, we show that rGNBP1 has a hydrolyzing activity against Lys-PG, which is enhanced in the presence of rPGRP-SA. We analyzed by mass spectrometry the majority of the products of this hydrolysis, which were released in the supernatant. These were identified as muropeptides demonstrating the muramidase-like activity of GNBP1. Moreover, when injected, the hydrolysis products were able to activate AMP expression in wild-type but not in PGRP-SA mutant flies. Additionally, the two proteins physically interact and this interaction is enhanced in the presence of GNBP1-hydrolyzed PG or highly purified PG fragments. These PG fragments are bound by rPGRP-SA and binding depends on the state of muropeptide polymerization and the presence of the nonreducing end of the terminal muramic acid. The above put forward a model whereby pattern recognition of Gram-positive PG is achieved by a GNBP1/PGRP-SA complex. Results To characterize the molecular events during Gram-positive PG sensing, we expressed and purified PGRP-SA and GNBP1 in a baculovirus insect cell culture system. Optimal expression conditions were determined for both proteins (see Materials and methods for details). Using a two-step chromatographic procedure, namely Ni-NTA affinity chromatography and gel filtration, rPGRP-SA and rGNBP1 were purified. The recombinant proteins were subjected to N-terminal protein sequencing and the identity of a mature form of purified rPGRP-SA lacking the signal peptide was confirmed (Figure 1A, lane 1). Similarly, the recombinant GNBP1 was established to be the mature protein with the signal peptide removed (Figure 1A, lane 2). Characterization of the purified proteins by analytical ultracentrifugation (AUC; see Materials and methods) showed that, in solution, rPGRP-SA was present as a monomer. Interestingly, rGNBP1 was able to form homodimers (Figure 1B). Figure 1.Protein expression, functional assays and AUC analysis. (A) Purified rPGRP-SA (predicted MW 20 kDa) and rGNBP1 (predicted MW 60 kDa) were analyzed on a 12% reducing SDS–PAGE gel. The identity of both proteins was verified by N-terminal protein sequencing (protein and nucleic acid chemistry service facility, Department of Biochemistry, University of Cambridge). As shown, the ∼20 amino-acid signal peptide was cleaved in both cases and the mature, secreted product detected. rGNBP1 and rPGRP-SA are biologically functional in vivo. (B) Analytical ultracentrifugation (AUC) analysis. Majority of rPGRP-SA appeared in a peak with a sedimentation coefficient (S) of 1.6, indicating PGRP-SA to be monomeric in solution. rGNBP1 showed two peaks at 2.8 and 4.2, respectively. The higher S species corresponds to a potential protein dimer of GNBP1 at higher concentrations in solutions. AUC of two proteins mixed showed a broad shoulder peak at an even higher S, suggesting a weak protein–protein interaction between the two proteins. The AUC curves for rPGRP-SA, rGNBP1 and the protein mixtures are shown in black, blue and purple, respectively. (C) Injection of 20 ng rGNBP1 is able to restore Drosomycin expression as assayed by Northern blot after Ml challenge in GNBP1 mutant flies. Injection of rGNBP1 does not activate any response in the absence of challenge. Wild-type (WT) flies and GNBP1osi flies infected with Ml were used as control. PBS injections were performed in WT flies as injection controls. (D) 10 ng injection of rPGRP-SA restores the GFP fluorescence in PGRP-SA mutant flies carrying a Drosomycin-GFP reporter gene after Ml challenge. Wild-type flies carrying the same reporter gene were used as controls. Again injection of the recombinant protein without challenge does not trigger Drs-GFP. Download figure Download PowerPoint We tested the in vivo activity of the purified recombinant proteins by injecting them into the respective mutant flies. We used as an assay the expression of the AMP gene drosomycin (drs), a transcriptional target of the Toll pathway (Filipe et al, 2005). Expression of drs was restored in GNBPosi mutant flies that were injected with 20 ng or rGNBP1 protein and subsequently infected by the Gram-positive bacterium Micrococcus luteus (Ml; Figure 1C). The recombinant PGRP-SA was injected into homozygous PGRP-SAseml mutant flies carrying a drs-GFP transgene. Injection of 10 ng rPGRP-SA into the mutant flies restored GFP expression following infection by Ml (Figure 1D). Taken together, these results indicate that both recombinant proteins are biologically functional. GNBP1 binds to and hydrolyzes Lys-type PG We have previously speculated that GNBP1 is able to act enzymatically on Lys-type PG, as hemolymph from GNBP1 mutant flies is not able to provoke a reduction in OD measurements of a blood/PG mixture, in contrast to wild-type flies where PG was hydrolyzed (Filipe et al, 2005). Reintroduction of GNBP1 through the expression of a UAS-transgene restored the observed activity (Filipe et al, 2005). Based on these results, we tested whether rGNBP1 was able to hydrolyze Lys-type PG. Recombinant GNBP1 (at 0.5 μM) was indeed able to hydrolyze PG from Ml and Enterococcus faecalis but not Staphylococcus aureus or Bacillus subtilis (Figure 2A and data not shown). As shown in Figure 2A, this activity is enhanced in the presence of rPGRP-SA (at 0.5 μM). These results indicate the cooperation of the two proteins and underscore the enzymatic ability of GNBP1 against certain types of Lys-PG. This selectiveness of GNBP1 as an enzyme correlates with the ability of rGNBP1 to bind some Lys-PG molecules and not others as denoted in Figure 2B. In addition, a limited degradation of PG in the presence of PGRP-SA was also observed (Figure 2A). It is interesting to note here that PGRP-SA exhibited a broader capacity of PG binding, as it could also bind DAP-type PG from Bacillus subtilis (Figure 2B). Figure 2.rGNBP1 binds and hydrolyzes PG with rPGRP-SA, generating an immunostimulatory PG in GNBP1 mutant flies, but not PGRP-SA mutant flies. (A) The hydrolytical assay was performed with rGNBP1 and/or rPGRP-SA in a sodium phosphate buffer (pH5.5) at 37°C. OD absorbance at 600 nm was taken at 0, 12, 24 and 36 h. In a 1:1 molar ratio, PG from Ml can be degraded more efficiently in the presence of both proteins than rGNBP1 alone. (B) Both rGNBP1 and rPGRP-SA bind to PG. In all, 20 μg of rGNBP1 or rPGRP-SA was used as input for the binding assay (see Materials and methods). Briefly, after incubation with the recombinant proteins, PG from different bacterial strains was removed from the reaction by centrifugation, washed and the association with recombinant proteins analyzed by SDS–PAGE. Recombinant GNBP1 and rPGRP-SA both bind to Ml PG (Lys-type), whereas rPGRP-SA exhibited significant binding affinity for Bacillus PG (DAP-type) and S. aureus PG (Lys-type). GNBP1 did not bind to these PGs. (C) GNBP1 but not PGRP-SA mutant flies respond to the rGNBP1-hydrolyzed PG (denoted as PG*). Intact PG or PG* were injected into flies at 5 mg/ml. Wild-type or mutant flies injected with PBS, PBS with 1% SDS (used for denaturing rGNBP1), intact PG and intact PG in 1% SDS were performed as controls. No difference was observed in the expected results when intact PG or intact PG in 1% SDS was injected to wild-type or mutant flies (this figure and data not shown). Injection of rGNBP1 in GNBP1-deficient flies before PG injection restores drosomycin expression to wild-type levels (lane rGNBP1+PG). Measurements of drosomycin expression were carried out 24 h following injection. Upper panel: Drosomycin probe (Drs). Lower panel: RP49 probe as internal RNA loading control. (D) Recombinant GNBP1 but not rPGRP-SA was able to release small PG fragments comparable to mutanolysin. Chromatography of the hydrolytically products after HPLC from top to bottom: PG after PGRP-SA treatment, PG alone, PG after rGNBP1 digestion and PG after mutanolysin digestion as control. Released small PG fragments that had their molecular weight determined by MALDI-MS are indicated by arrows. Download figure Download PowerPoint To test for the inflammatory properties of the reaction products following the GNBP1-mediated hydrolysis, we injected the supernatant (after precipitation of the remaining PG and heat inactivation of GNBP1) to adult flies. We monitored activation by analyzing the expression of drs. As shown in Figure 2C, induction of drs is achieved in both wild-type and GNBP1osi mutants but not in PGRP-SA-deficient flies. These data indicate that the products of the reaction have inflammatory properties and that the limiting factor for the step following GNBP1-mediated PG hydrolysis is recognition by PGRP-SA. Moreover, injection of Ml PG digested by GNBP1 to various extends resulted in a correlation between the level of hydrolysis and drs expression (Supplementary Figure S1). Max levels of drs were observed when OD of PG was reduced at 80% something that happens between 24 and 36 h. The products that resulted from PG hydrolysis by GNBP1 were as capable to promote drs induction as purified polymerized muropeptides (Filipe et al, 2005). We asked therefore, whether GNBP1 could be releasing muropeptides into the supernatant when incubated with PG. To this end, we analyzed the supernatant of the reaction mixture by high-pressure liquid chromatography (HPLC) (Figure 2D). Two major peaks could be identified eluting at 22 and 26 min, similarly to what was observed when PG was digested by mutanolysin (arrows in Figure 2D). We collected the fractions eluting at 22 and 26 min and determined their molecular weight by Mass Spectrometry (MS). For both GNBP1 and mutanolysin digests, the obtained molecular weights corresponded to a monomeric muropeptide with only one terminating D-Ala residue (m/z 955) or with two terminating D-Ala residues (m/z 1026) (SRF, unpublished results). The ability of GNBP1 to release monomeric muropeptides from PG, similarly to mutanolysin, thus confirmed a muramidase-like activity for GNBP1. However, this activity was less efficient than that of mutanolysin, as only monomeric muropeptides were released indicating that GNBP1 attacks bonds not as frequently or does it at specific sites of Ml's PG. The released monomeric muropeptides should not be active because we have shown that monomeric muropeptides are unable to trigger an immune response (Filipe et al, 2005). Instead, it is conceivable that the processed remaining PG should have an increased number of reducing ends that result from the infrequent cuts of GNBP1. The specific site where GNBP1 cleaves and the structural requirements for an efficient cut are under current investigation (SRF and PL, in preparation). PG enhances the physical interaction between GNBP1 and PGRP-SA Data from AUC pointed to the formation of a higher molecular weight complex between the two proteins. Briefly, when rPGRP-SA and rGNBP1 were mixed at 5.9 and 2.3 μM, respectively, we observed the formation of a higher molecular weight complex while the rGNBP1 dimer peak disappeared (compare the blue and purple lines in Figure 1B). Given this and the results described previously, we sought to determine whether we could quantify this interaction in a purified system. We addressed the question using the technique of surface plasmon resonance (Biacore). For these experiments, we immobilized PGRP-SA on a sensor chip and measured the relative response following injection of GNBP1. A direct interaction between PGRP-SA and GNBP1 could be reproducibly detected (Figure 3A). By using serial dilutions of pure rGNBP1 injections, we were able to quantify this interaction (Figure 3B). We confirmed binding by doing the reciprocal experiment with immobilized GNBP1 and injection of rPGRP-SA (Figure 3B). These results indicate a physical interaction between the two proteins with a KD in the low micromolar range. This interaction was enhanced if before injection GNBP1 was mixed with Methylobacter luteus PG or S. aureus muropeptides and then passed over the PGRP-SA sensor chip (Figure 3C left panel for PG and right panel for muropeptides). A summary of the dissociation constants measured for all interactions is shown in Figure 3B. Specifically, mixing different dilutions of a tetrameric muropeptide (7.5–100 nM) with GNBP1 and subsequent passing through the PGRP-SA chip augmented the interaction between the two proteins from a calculated KD of 21 μM (PGRP-SA/GNBP1) to that of 15 μM (PRGP-SA/GNBP1/Muropeptide tetramer; Figure 3D). The reverse, namely mixing PGRP-SA with Ml PG and passing over the GNBP1 sensor chip did not have the same effect (data not shown). This points towards the existence of a tripartite complex as well as a specific mode of sensing involving the two proteins and PG, whereby GNBP1 interacts with PG facilitating the binding of PGRP-SA (see discussion). The structure of the tetrameric muropeptide is shown in Figure 4A. Figure 3.rGNBP1 and rPGRP-SA interact and hydrolyzed PG or muropeptides can enhance the strength of this interaction. (A) Surface plasmon resonance (Biacore) was used to study the interaction between rGNBP1 and rPGRP-SA. (A) rPGRP-SA was immobilized on a CM5 sensor chip and serial dilutions of rGNBP1 were injected over the sensor chip. With the increase of protein concentration, the read-out in response units (RU) increased accordingly. The reverse experiment namely immobilization of rGNBP1 on a CM5 sensor chip and injection of serial dilutions of rPGRP-SA gave similar results (data not shown). Dissociation constants (KDs) for both experiments are indicated in panel (B). (B) A summary of KDs based on a series of binding curves for the Biacore experiments described in this figure as well as in Figure 4 (PGRP-SA/muropeptide interaction). All KDs were calculated using the BIAevulation software (see Materials and methods). Three binding curves were used for each KD measurement. (C) Hydrolyzed PG (left panel) or muropeptides (right panel) enhanced the binding between rGNBP1 and rPGRP-SA. In this experiment, rPGRP-SA was coated on the sensor chip. (D) Quantification of the interaction of rPGRP-SA and rGNBP1 in the presence of muropeptides, using serial dilutions of a highly purified tetrameric muropeptide from S. aureus. As in (C), rPGRP-SA was coated on the sensor chip. The calculated KD is presented in (B). Note that from a KD of 20 μM for the interaction of rPGRP-SA/rGNBP1, there is a decrease in the calculated KD (16 μM) and therefore an enhancement of interaction when before injection rGNBP1 is mixed with the tetrameric muropeptide. Download figure Download PowerPoint Figure 4.Polymerized muropeptides are able to shift rPGRP-SA to a higher molecular weight and rPGRP-SA is the protein that responds to muropeptide but not rGNBP1. (A) Chemical structure of unreduced and reduced muropeptide tetramer from S. aureus. (B) Polymerized muropeptides shifted rPGRP-SA to a higher molecular weight on a native gel. In each lane, 10 μg of rPGRP-SA were loaded and incubated with 25 μl of different biotinylated muropeptides (0.1 mg/ml) for 3 h at room temperature before loading onto the gel. After separation, proteins were transferred to PVDF as described in Materials and methods and probed with a streptavidin–peroxidase conjugate. Muropeptides with the muramic acid reduced (shown in red in A) were also investigated for their ability to bind to rPGRP-SA. Whereas dimeric muropeptides only bind rPGRP-SA in an unreduced state, muropeptide tetramers bind rPGRP-SA in both reduced and unreduced states. Monomeric muropeptides are unable to bind to the protein. (C) Although both reduced and unreduced tetramers bind to PGRP-SA, only the latter induce immune response confirming the significance of the free reducing end of the muramyl acid for recognition (Filipe et al, 2005). Moreover, mixing of reduced tetramers with their unreduced counterparts at a ratio of 1 reduced to 4 unreduced molecules had a severe effect in drs expression levels. This result indicates that reduced tetramers act as competitors for recognition by binding to PRGP-SA and thus diluting the elicitor effect of unreduced tetramers. (D) Biotinylated tetrameric and dimeric muropeptides were immobilized on a streptavidin-coated Biacore sensor chip and rGNBP1 or rPGRP-SA injected. Whereas rGNBP1 does not bind to either muropeptide, rPGRP-SA interacts weakly with both dimer and tetramer. (E) Quantification of the interaction shown in (D) using a tetrameric muropeptide coated on the sensor chip and injecting a dilution series of rPGRP-SA. The calculated KD is shown in Figure 3B. Download figure Download PowerPoint PGRP-SA binds to polymerized muropeptides The data presented so far indicate that GNBP1 is able to present PG to PGRP-SA in a processed form. To study the binding requirements of PGRP-SA and its affinity for fragments of Lys-PG, we sought to investigate whether PGRP-SA was able to bind highly purified muropeptides from S. aureus. We tested this by mixing the protein with different, biotin labeled, muropeptides and then visualized the interaction on native protein gels followed by immunoblotting (see Materials and methods). We had previously identified that a muropeptide dimer as the minimal structure activating the Toll pathway when injected to wild-type adults (Filipe et al, 2005). However, this structure could not trigger AMP gene expression in a PGRP-SAseml mutant background (Filipe et al, 2005). In agreement with our previous results, we found that rPGRP-SA is indeed able to bind dimeric muropeptides but not monomers. Moreover, the free end of the muramic acid is important for binding as reducing it prevents binding of PGRP-SA (Figure 4B) and it renders the dimer unable to activate the Toll pathway in flies (Filipe et al, 2005). As shown in Figure 4B, PGRP-SA also exhibits a binding affinity for tetrameric muropeptides. It was surprising that the reduced tetrameric muropeptides were able to interact with PGRP-SA, as we have previously shown that a reduced tetramer is unable to trigger an inflammatory response in Drosophila (Filipe et al, 2005). However, this binding did not lead to signaling. Mixing of reduced and unreduced tetramers in a ratio of 1:4 molecules significantly reduced drs activation when injected to wild-type flies (Figure 4C; see Figure 4A for the structural differences between unreduced/reduced tetramers). This indicates that the reduced and unreduced tetrameric muropeptides are competing for the same receptor and that the free reducing end is still important for an inflammatory response. In addition, this points to different requirements for binding between dimeric and tetrameric muropeptides (as reduced tetramers bind PGRP-SA but reduced dimers do not). These results along with the recent 3D structural data for PGRP-SA (Chang et al, 2004; Reiser et al, 2004) suggest that the stoichiometry of the interaction is one PGRP-SA molecule for every muropeptide dimer moiety and that as muropeptide polymerization increases more protein molecules are recruited. Cooperative binding of PGRP-SA molecules to the tetrameric structure may facilitate the interaction between the receptor and this muropeptideas opposed to the dimereven in a reduced state. Co-crystallization studies of rPGRP-SA and reduced or unreduced dimer and tetramer will eventually provide a definite answer (NJ Gay unpublished). Finally, the interaction between rPGRP-SA and muropeptides was further confirmed using Biacore, where different muropeptides were passed through a PGRP-SA-coated chip (Figure 4D). This interaction was quantified with a KD of 30 μM using the tetrameric muropeptide from S. aureus (Figure 4E). Of note is the fact that GNBP1 was never seen to interact with any of these structures. Discussion In recent years, genetic screens have revealed that in Drosophila, the Toll pathway is activated by distinct PRRs sensing PG upstream of the receptor itself (Michel et al, 2001; Gobert et al, 2003; Leulier et al, 2003; Pili-Floury et al, 2004) Until now however, the molecular recognition events that define the mechanism of Gram-positive-PG recognition remained elusive. In the present, we have analyzed the mode of sensing of two PRRs (PGRP-SA and GNBP1) needed in response to some classes of Gram-positive bacteria. Our results show that GNBP1 functions as an enzyme, which recognizes β-1,4 N-glucosaminoglycans such as PG and is able to hydrolyze and present PG to PGRP-SA. GNBP1 is part of the GNBP/β-1,3-Glucan recognition family of proteins, which comprises members from several insects (Zhang et al, 2003). These proteins contain an N-terminal glucan-binding domain and a C-terminal domain similar to β-1,3- and β-1,4-bacterial glucanases and a β-1,3-glucanase from the sea urchin Strongylocentrotus purpuratus (Yahata et
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