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Antioxidant activity of polysaccharide purified from Acanthopanax koreanum Nakai stems in vitro and in vivo zebrafish model

2015; Elsevier BV; Volume: 127; Linguagem: Inglês

10.1016/j.carbpol.2015.02.043

1879-1344

Min‐Cheol Kang, Seo-Young Kim, Eun-A. Kim, Ji‐Hyeok Lee, Youngsun Kim, Seok-Kyu Yu, Jun Byoung Chae, In-Hu Choe, Jin Hun Cho, You‐Jin Jeon,

Moringa oleifera research and applications

In this study, the antioxidative effects of a purified polysaccharide isolated from the stems of Acanthopanax koreanum Nakai (ASP) on hydrogen peroxide-induced oxidative stress was investigated both in vitro and in vivo using a zebrafish model. A. koreanum Nakai stem was hydrolyzed using five carbohydrases and five proteases for the enzyme-assistant extraction. Of the enzyme-assistant extracts, the Protamex extract exhibited the highest yield and a potent scavenging activity against free radicals. Ethanol-added separation and anion exchange chromatography were conducted to identify the active polysaccharide. The purified polysaccharide significantly scavenged hydrogen peroxide and reduced hydrogen peroxide-induced cell death in Vero cells and in zebrafish. The results reveal that ASP is a useful antioxidant polysaccharide and might be available for relevant industrial applications.