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Determination of endogenous thiols and thiol drugs in urine by HPLC with ultraviolet detection

2009; Elsevier BV; Volume: 877; Issue: 28 Linguagem: Inglês

10.1016/j.jchromb.2009.03.038

1873-376X

Krzysztof Kuśmierek, Grażyna Chwatko, Rafał Głowacki, Edward Bald,

Folate and B Vitamins Research

Analysis of urine for endogenous thiols and thiol drugs content by HPLC with ultraviolet detection is addressed. Other methodologies for detection and determination of thiols in urine are only mentioned. Outline of metabolism, role of main biological thiols in physiological and pathological processes and their reference concentrations in urine are presented. In particular, urine sample preparation procedures, including reduction of thiol disulfides, chemical derivatization and reversed-phase HPLC separation steps are discussed. Some experimental details of analytical procedures for determination of endogenous thiols cysteine, cysteinylglycine, homocysteine, N-acetylcysteine, thioglycolic acid; and thiol drugs cysteamine, tiopronin, d-penicillamine, captopril, mesna, methimazole, propylthiouracil and thioguanine are reviewed.