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Developmental expression of Shisa-2 in Xenopus laevis

2006; University of the Basque Country; Volume: 50; Issue: Next Linguagem: Inglês

10.1387/ijdb.062141as

1696-3547

Ana-Cristina Silva, Mário Filipe, Marta Vitorino, Herbert Steinbeißer, José António Belo,

Congenital heart defects research

Shisa is an antagonist of Wnt and FGF signaling, that functions cell autonomously in the endoplasmic reticulum (ER) to inhibit the post-translational maturation of Wnt and FGF receptors. In this paper we report the isolation of a second Xenopus shisa gene (Xshisa-2) . Xenopus Shisa-2 shows 30.7% identity to Xshisa. RT-PCR analysis indicated that Xshisa-2 mRNA is present throughout early development and shows an increased expression during neurula and tailbud stages. At neurula stages Xenopus shisa-2 is initially expressed in the presomitic paraxial mesoderm and later in the developing somites. The expression profiles and pattern of Xshisa and Xshisa-2 differ significantly. During gastrulation only Xshisa mRNA is present in the Spemann-Mangold organizer and later on becomes restricted to the neuroectoderm and the prechordal plate.