Changes in haemolymph proteins during the metamorphosis of both sexes and castes of polygynous Formica rufal. (ins. hym)
1975; Elsevier BV; Volume: 52; Issue: 3 Linguagem: Inglês
10.1016/0305-0491(75)90147-9
ISSN0305-0491
AutoresGerhard Schmidt, Walter Schwankl,
Tópico(s)Animal Behavior and Reproduction
Resumo1. During the cocoon period the haemolymph proteins of three populations of polygynous Formica rufa were fractionated on polyacrylamide gels by disk-electrophoretic methods. The protein fractions were stained by Aniline Blue Black and determined quantitatively by a densitometric technique using serum albumin as standard protein. 2. In both sexes and castes we found 14 fractions, from them; nine were considered as major bands (f1a, f1b, f2, f3, f5, f6, f8, f11 and f14 in the sense of anodic runnings). 3. All fractions changed independently during the metamorphosis. The proteins of the major bands amounted to 70–98%. 4. No distinct differences between sexual forms and castes could be demonstrated. When differences are present they are probably caused by the different organ development. 5. The total protein content had a minimum during pupation and amounted to 2% (g/100 ml) in the later pupal stage. After this maximum it decreased strongly to 0·42% on the hatching point.
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