A fluorometric assay for β-phenylethylamine in rat brain
1976; Elsevier BV; Volume: 75; Issue: 1 Linguagem: Inglês
10.1016/0003-2697(76)90070-1
ISSN1096-0309
Autores Tópico(s)Biochemical Analysis and Sensing Techniques
ResumoAbstract β-Phenylethylamine was found to react with ninhydrin in the presence of l -leucyl- l -alanine to yeild a highly fluorescent compound which seems to be specific for β-phenylethylamine, phenylalanine, and 5-hydroxytryptamine. The amount of β-phenylethylamine in rat brain could be determined by this reaction after its separation from phenylalanine and 5-hydroxytryptamine by n -heptane extraction. The content of endogenous β-phenylethylamine in rat brain was 5 ng/g of wet tissue. Administration of pargyline caused more than a 10-fold increase in brain β-phenylethylamine. After the injection of both pargyline and phenylalanine, β-phenylethylamine was increased about 100-fold. This increase was reduced by prior injection of reserpine, while enhanced by that of calcium fusarate.
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