Membrane Protein Stoichiometry Determined from the Step‐Wise Photobleaching of Dye‐Labelled Subunits
2007; Wiley; Volume: 8; Issue: 9 Linguagem: Inglês
10.1002/cbic.200600474
ISSN1439-7633
AutoresSomes K. Das, Manjula Darshi, Stephen Cheley, Mark I. Wallace, Hagan Bayley,
Tópico(s)Lipid Membrane Structure and Behavior
ResumoBody count.In a generally applicable approach, the number of subunits in fluorescently-labelled protein complexes has been determined by counting photobleaching steps from individual molecules (see figure). The distribution of steps in the pore-forming toxins α-haemolysin and leukocidin indicate seven subunits for α-hemolysin, and four LukF and four LukS subunits for leukocidin. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2007/z600474_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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