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Enzyme assays for high-throughput screening

2004; Elsevier BV; Volume: 15; Issue: 4 Linguagem: Inglês

10.1016/j.copbio.2004.06.008

1879-0429

Jean‐Philippe Goddard, Jean‐Louis Reymond,

Advanced biosensing and bioanalysis techniques

Assaying enzyme-catalyzed transformations in high-throughput is crucial to enzyme discovery, enzyme engineering and the drug discovery process. In enzyme assays, catalytic activity is detected using labelled substrates or indirect sensor systems that produce a detectable spectroscopic signal upon reaction. Recent advances in the development of high-throughput enzyme assays have identified new labels and chromophores to detect a wide range of enzymes activities. Enzyme activity profiling and fingerprinting have also been used as tools for identification and classification, while microarray formats have been devised to increase throughput.