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Preparation and Evaluation of Slurry-Packed Liquid Chromatography Microcolumns with Inner Diameters from 12 to 33 μm

1996; American Chemical Society; Volume: 68; Issue: 7 Linguagem: Inglês

10.1021/ac950682m

1520-6882

Showchien Hsieh, James W. Jorgenson,

Protein purification and stability

Fused silica capillary liquid chromatography columns with inner diameters between 12 and 33 μm were slurry packed with 5 μm octadecylsilane-modified silica particles. Column efficiencies and van Deemter coefficients were compared. A linear decrease of the A term as column diameter was decreased was the most significant contributor to a lower overall plate height at the optimum velocity.