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The biosynthesis of 11-ketotestosterone by the testis of the Siamese fighting fish Betta splendens regan (anabantoidei, belontiidae)

1987; Elsevier BV; Volume: 66; Issue: 1 Linguagem: Inglês

10.1016/0016-6480(87)90359-5

1095-6840

Thomas Leitz, Rudolf Reinboth,

Steroid Chemistry and Biochemistry

Testicular tissues of the Siamese fighting fish were incubated with [14C]pregnenolone for 10, 20, 30, 50, 80, and 120 min, and with [14C]progesterone, [14C]11β-hydroxyandrostenedione, [14C]11β-hydroxytestosterone, and [14C]androstenetrione for 120 min. 11-Ketotestosterone was the main metabolite in all 120-min incubations. No 11-oxygenated C21 steroids were found as metabolites of either pregnenolone or progesterone. The biosynthesis of 11-ketotestosterone proceeded through both the Δ5- and the Δ4- pathways as judged from the shape of the yield-time curves of the metabolites of pregnenolone. 11-Ketotestosterone formation from 11-oxygenated precursors increased in the order 11β-hydroxytestos-terone < 11β-hydroxyandrostenedione < androstenetrione.