Benoxaprofen Photosensitization of Cell Membrane Disruption
1984; Elsevier BV; Volume: 82; Issue: 3 Linguagem: Inglês
10.1111/1523-1747.ep12260034
ISSN1523-1747
AutoresIrene E. Kochevar, Karen Wujek Hoover, Margaret Gawienowski,
Tópico(s)Animal testing and alternatives
ResumoBenoxaprofen (BXP) is a nonsteroidal anti-inflammatory drug which causes cutaneous phototoxicity. Because the in vivo phototoxicity may involve photosensitized damage to mast cell membranes, the mechanism for photosensitized damage was studied in a single model system, the red blood cell. Oxygen-dependent and oxygen-independent mechanisms for membrane disruption were detected. Oxygen-dependent lysis was not quenched by superoxide dismutase and was quenched only at high sodium azide concentrations. A two-step mechanism is proposed involving initial photodecarboxylation of BXP to form a lipophilic photoproduct which subsequently photosensitizes membrane damage. Human serum albumin at 0.03% totally inhibited BXP-photosensitized lysis.
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