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BIBTEX RIS

Kinetic studies of recombinant rabies virus glycoprotein (RVGP) cDNA transcription and mRNA translation in Drosophila melanogaster S2 cell populations

2013; Springer Science+Business Media; Volume: 65; Issue: 5 Linguagem: Inglês

10.1007/s10616-012-9522-6

1573-0778

Renato Mancini Astray, Soraia Attie Calil Jorge, Marcos Alexandre Nobre Lemos, Adriana Y. Yokomizo, Vera Lucia Lopes Boldorini, Ana L.P. Puglia, Orlando Ribeiro, Carlos Alberto de Bragança Pereira,

Toxin Mechanisms and Immunotoxins

Recombinant rabies virus glycoprotein (RVGP) was expressed in cell membranes of stably transfected Drosophila S2 cells using constitutive and inducible promoters. Although with quantitative differences of RVGP expression in both systems, the cDNA transcription, as evaluated by relative RVGP mRNA levels measured by qRT-PCR, sustained the amount of RVGP producing cells and the RVGP volumetric (ΠRVGP) productivity. At the transition to the stationary cell growth phase, once the cell culture slowed down its rate of multiplication, an accumulation of RVGP mRNA and RVGP was clearly observed in both cell populations. Nevertheless, cell cultures performed under sub-optimal temperatures indicated that an envisaged increase in the RVGP production is not only dependent on cell growth rate, but essentially on optimal cell metabolic state.