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CCQM-K86/P113.1: Relative quantification of genomic DNA fragments extracted from a biological tissue

2012; IOP Publishing; Volume: 49; Issue: 1A Linguagem: Inglês

10.1088/0026-1394/49/1a/08002

1681-7575

Philippe Corbisier, Vincent Savolainen, H. Schimmel, A-M Kortekaas, Stefanie Trapmann, M Burns, Claire Bushell, Müslüm Akgöz, Sema Akyürek, Lü Dong, B. Fu, L. Zhang, J. Wang, Melina Pérez Urquiza, Jaimes Bautista, Aritz Pérez Ruiz de Garibay, B W Fuller, Anna Baoutina, Lina Partis, Kerry R. Emslie, M.J. Holden, W Y Chum, H. H. Kim, Nittaya Phunbua, Mojca Milavec, Jana Žel, M. S. Vonsky, L A Konopelko, T L T Lau, Bing Yang, M H K Hui, Amanda Yu, D Viroonudomphol, C Prawettongsopon, Kanjana Wiangnon, Reona Takabatake, Kazumi Kitta, Mamoru Kawaharasaki, H.C. Parkes,

Wheat and Barley Genetics and Pathology

Key comparison CCQM-K86 was performed to demonstrate and document the capacity of interested national metrology institutes (NMIs) and designated institutes (DIs) in the determination of the relative quantity of two specific genomic DNA fragments present in a biological tissue. The study provides the support for the following measurement claim: "Quantification of the ratio of the number of copies of specified intact sequence fragments of a length in the range of 70 to 100 nucleotides in a single genomic DNA extract from ground maize seed materials".