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Staurosporine inhibits the soluble and membrane-bound protein tyrosine kinases of human neutrophils

1991; Elsevier BV; Volume: 178; Issue: 2 Linguagem: Inglês

10.1016/0006-291x(91)90124-p

1090-2104

John A. Badwey, Richard W. Erickson, John T. Curnutte,

Nitric Oxide and Endothelin Effects

Superoxide production by neutrophils triggered with a chemotactic peptide or a phorbol ester is inhibited by the protein kinase antagonists staurosporine or 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7). We evaluated the effects of these antagonists on the protein tyrosine kinases and protein kinase C activities of neutrophils. Staurosporine completely inhibited all of these enzymes, whereas 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine was only substantially effective against protein kinase C. Thus, if a protein tyrosine kinase is involved in superoxide production, it is likely to function with a second kinase sensitive to 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine.