Temperature-Sensitive R Plasmid Obtained from Naturally Isolated Drug-Resistant Vibrio cholerae (Biotype El Tor)
1977; American Society for Microbiology; Volume: 11; Issue: 1 Linguagem: Inglês
10.1128/aac.11.1.13
ISSN1098-6596
AutoresTakeshi Yokota, Shōgo Kuwahara,
Tópico(s)Antibiotic Resistance in Bacteria
ResumoA temperature-sensitive fi − R plasmid, pJY1, which confers resistance to chloramphenicol, streptomycin, and sulfonamide, was found in a drug-resistant strain of Vibrio cholerae (biotype El Tor) isolated in the Philippines in 1973. The R plasmid is temperature sensitive for transferability but not for stability in the hosts. pJY1 is stably maintained either in V. cholerae or in Escherichia coli at a range of culture temperatures from 27 to 42°C. Compatibility tests with reference R plasmids revealed that pJY1 belongs to the J group. Growth of V. cholerae carrying pJY1 is poor on TCBS (thiosulfate-citrate-bile-sucrose) selective plates when the microbe is subcultured in nutrient broth at temperatures higher than 37°C.
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