Determination of anthocyanins in blood orange juices by HPLC analysis
2000; Elsevier BV; Volume: 23; Issue: 1 Linguagem: Inglês
10.1016/s0731-7085(00)00269-7
ISSN1873-264X
AutoresLuigi Mondello, Antonella Cotroneo, Giacinto Errante, Giovanni Dugo, Paola Dugo,
Tópico(s)Plant Gene Expression Analysis
ResumoDetermination of anthocyanins in fresh and concentrated juices can be a parameter for the assessment of authenticity and quality of blood orange juices. This work reports an HPLC/UV-Vis method developed for quantitative determination of anthocyanins in blood orange juices, by using a calibration curve obtained for standard cyanidin-3-glucoside. Samples analysed have been obtained from fruits of different trees (one for each of the varieties: 'Moro', 'Tarocco', 'Sanguinello' and 'Sanguinello nocellare') harvested about every 15 days during the 1998 productive season. Seasonal variation has been also evaluated. HPLC results were compared with spectrophotometric measurements, using a calibration curve obtained for cyanidin-3-glucoside solutions. The two methods showed good agreement, but the results obtained greatly differed with the data reported in the literature.
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