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Tissue–response to calcium‐bonded titanium surface

2010; Wiley; Volume: 95A; Issue: 1 Linguagem: Inglês

10.1002/jbm.a.32763

1552-4965

Lei Zhang, Yasunori Ayukawa, Racquel Z. LeGeros, Shigeki Matsuya, Kiyoshi Koyano, Kunio Ishikawa,

Orthopaedic implants and arthroplasty

Abstract Our previous study demonstrated that calcium‐bonded titanium surface (Ca‐Ti) can be obtained by hydrothermal reaction between titanium (Ti) and CaCl 2 and that bone‐apatite like formation was observed after immersion in simulated body fluid. The purpose of the study was to determine the in vivo response to Ca‐Ti surface using a rodent tibia model. Cylinders of commercially pure Ti were divided into three groups: (1) untreated group; (2) NaOH+hTi group: soaked in 5 mol/L NaOH solution at 60°C then heated at 400°C for 1 h; and (3) Ca‐Ti group: hydrothermally treated in the presence of 10 mmol/L CaCl 2 at 200°C for 24 h. The cylinders implanted in surgically created defects in tibias of 8‐week old male Wistar rats were retrieved after 1, 2, and 4 weeks. Histomorphometric evaluations were made on stained decalcified thin sections. Results showed that at 1, 2, and 4 week after implantation, respectively, bone contact was 55.2 ± 16.4%, 88.1 ± 9.9%, and 96.1 ± 4.8% for Ca‐Ti implants, 5.7 ± 5.3%, 19.9 ± 1.2%, 57.4 ± 4.8% for untreated; and 27.2 ± 0.7%, 70.9 ± 7.7%, and 96.0 ± 5.1% for NaOH+hTi implants. These results suggest that hydrothermal treatment with CaCl 2 provides a bioactive Ca‐Ti bonded surface that allows bone formation greater than that obtained with NaOH+heat treated Ti surfaces. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.