Portal de Periódicos da CAPES

Molecular distribution sensing in a fluorescence resonance energy transfer based affinity assay for glucose

2001; Elsevier BV; Volume: 57; Issue: 11 Linguagem: Inglês

10.1016/s1386-1425(01)00475-9

1873-3557

Olaf J. Rolinski, David J. S. Birch, Lydia J. McCartney, John C. Pickup,

Electrochemical sensors and biosensors

A newly developed method for determining molecular distribution functions is applied to a widely researched glucose affinity sensor. The reduction in fluorescence resonance energy transfer (FRET) to a malachite green (MG)–dextran complex from allophycocyanin (APC) bound to concanavalin A (ConA) due to displacement of the complex by glucose from ConA provides the basis of the assay. The higher sensitivity and specificity of a new approach to fluorescence decay analysis, over the methods based on conventional Förster-type models, is demonstrated and critical parameters in competitive binding FRET sensing derived.