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Activated macrophages are responsible for the tumor-inhibitory effect in mice receiving intravenous injection of OK-432

1984; Wiley; Volume: 33; Issue: 2 Linguagem: Inglês

10.1002/ijc.2910330217

1097-0215

Motoo Saito, Masaki Nanjo, Etsuko Aonuma, Tetsuo Noda, Ichiro Nakadate, Takusaburo Ebina, Nakao Ishida,

Immune cells in cancer

International Journal of CancerVolume 33, Issue 2 p. 271-276 Article Activated macrophages are responsible for the tumor-inhibitory effect in mice receiving intravenous injection of OK-432 Motoo Saito, Motoo Saito Department of Bacteriology, Tohoku University School of Medicine, 2–1 Seiryo-machi, Sendai 980, JapanSearch for more papers by this authorMasaki Nanjo, Masaki Nanjo Department of Bacteriology, Tohoku University School of Medicine, 2–1 Seiryo-machi, Sendai 980, JapanSearch for more papers by this authorEtsuko Aonuma, Etsuko Aonuma Department of Bacteriology, Tohoku University School of Medicine, 2–1 Seiryo-machi, Sendai 980, JapanSearch for more papers by this authorTetsuo Noda, Tetsuo Noda Department of Bacteriology, Tohoku University School of Medicine, 2–1 Seiryo-machi, Sendai 980, JapanSearch for more papers by this authorIchiro Nakadate, Ichiro Nakadate Department of Bacteriology, Tohoku University School of Medicine, 2–1 Seiryo-machi, Sendai 980, JapanSearch for more papers by this authorTakusaburo Ebina, Takusaburo Ebina Department of Bacteriology, Tohoku University School of Medicine, 2–1 Seiryo-machi, Sendai 980, JapanSearch for more papers by this authorNakao Ishida, Nakao Ishida Department of Bacteriology, Tohoku University School of Medicine, 2–1 Seiryo-machi, Sendai 980, JapanSearch for more papers by this author Motoo Saito, Motoo Saito Department of Bacteriology, Tohoku University School of Medicine, 2–1 Seiryo-machi, Sendai 980, JapanSearch for more papers by this authorMasaki Nanjo, Masaki Nanjo Department of Bacteriology, Tohoku University School of Medicine, 2–1 Seiryo-machi, Sendai 980, JapanSearch for more papers by this authorEtsuko Aonuma, Etsuko Aonuma Department of Bacteriology, Tohoku University School of Medicine, 2–1 Seiryo-machi, Sendai 980, JapanSearch for more papers by this authorTetsuo Noda, Tetsuo Noda Department of Bacteriology, Tohoku University School of Medicine, 2–1 Seiryo-machi, Sendai 980, JapanSearch for more papers by this authorIchiro Nakadate, Ichiro Nakadate Department of Bacteriology, Tohoku University School of Medicine, 2–1 Seiryo-machi, Sendai 980, JapanSearch for more papers by this authorTakusaburo Ebina, Takusaburo Ebina Department of Bacteriology, Tohoku University School of Medicine, 2–1 Seiryo-machi, Sendai 980, JapanSearch for more papers by this authorNakao Ishida, Nakao Ishida Department of Bacteriology, Tohoku University School of Medicine, 2–1 Seiryo-machi, Sendai 980, JapanSearch for more papers by this author First published: 15 February 1984 https://doi.org/10.1002/ijc.2910330217Citations: 45AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Mouse spleen cells, either pretreated in vitro with 100 U/ml of OK-432-induced IFN gamma for 18 h or obtained from mice 24 or 48 h after i.v. injection of OK-432(100 μg/mouse), were examined for their anti-tumor effect by Winn's neutralization assay against Meth-A tumor cells in BALB/c mice. Spleen cells treated in vitro or obtained in vivo 24 h after i.v. injection clearly neutralized the growth of admixed Meth-A cells. Two booster injections of 200 U of IFN gamma near the tumor site accelerated this neutralizating effect. In order to determine the effector subpopulation, inhibitory spleen cells were treated with either anti-Thy-1 monoclonal antibody plus complement, antiasialo GM1 serum plus complement or with adherence on plastic plates followed by Sephadex G-10 column treatment, The effector cell activity in Winn assay was lost only after the removal of macrophages through plastic plate adherence and Sephadex G-10 column treatment, but not after anti-Thy-1 or anti-asialo GM1 treatment, with either in vitro- or in vivo-treated spleen-cell populations. The growth of Meth-A cells was inhibited not only by these activated macrophages in Winn's assay, but also by adoptive transfer of OK-432-induced cytotoxic macrophages intralesionally 4 days after the implantation of 1 × 106 Meth-A cells. Our evidence suggests that the systemic action of OK-432 can be explained by the effect of induced. IFNgamma, through the activation of macrophages. References Bast, R. C., Bast, B. S., and Rapp, H. 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