A simple high performance liquid chromatography assay for monitoring plasma concentrations of tipranavir in HIV infected patients
2006; Elsevier BV; Volume: 832; Issue: 2 Linguagem: Inglês
10.1016/j.jchromb.2006.02.004
ISSN1873-376X
AutoresÉric Dailly, Véronique Reliquet, Caroline Victorri‐Vigneau, François Raffi, Pascale Jolliet,
Tópico(s)HIV Research and Treatment
ResumoA simple HPLC assay to determine plasma concentration of tipranavir is presented. A liquid/liquid extraction of the drugs in ethyl acetate/hexane from 250 microL of plasma is followed by a reversed phase isocratic HPLC assay with UV detection at 205 nm. The imprecision and inaccuracy are lower than 10%, the low limit of quantitation is 0.4 mg/L. Thus, this method can be used for therapeutic drug monitoring of tipranavir in HIV infected patients.
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