Highly sensitive differential pulse amperometric detection of second generation anti-tumor platinum compounds in HPLC effluents
1983; Springer Science+Business Media; Volume: 17; Issue: 6 Linguagem: Inglês
10.1007/bf02270669
ISSN1612-1112
AutoresW. J. F. van der Vijgh, H. B. J. van der Lee, Geert Postma, Herbert M. Pinedo,
Tópico(s)Advanced biosensing and bioanalysis techniques
ResumoA highly sensitive on-line amperometric detection of platinum compounds in HPLC effluents was possible with the use of a polarographic detector. For TNO-1 (=cis-1,1-di(aminomethyl)-cyclohexane Pt(II)chloride) a linear dynamic range of at least three decades could be obtained (0.034–108μg TNO-1/ml) with a detection limit of three times the noise signal at 0.7 ng TNO-1 (20ng Pt/ml). The sensitivity was 0.1 nA/ng TNO-1 (0.2nA/ng Pt). The within-day presicion was 1.1% at a concentration of 10.8μg TNO-1/ml (n=10). With this system the compatibility was measured of TNO-6 with infusion fluids. In 0.15 M NaCl, TNO-6 (=cis-1,1-di(aminomethyl)-cyclohexanePt(II)sulphate) was converted into TNO-1 within 40 minutes. Molecular changes of TNO-6 were also observed in a 5% aqueous glucose solution.
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