Evaluation of a direct radioimmunoassay for carcinoembryonic antigen
1978; Elsevier BV; Volume: 11; Issue: 4 Linguagem: Inglês
10.1016/s0009-9120(78)90417-4
ISSN1873-2933
AutoresT. A. McPherson, Maria Koch, James E. Coates,
Tópico(s)Lung Cancer Research Studies
ResumoA radioimmunoassay for CEA using commercially available reagents (CEA-Roche), which avoids the perchloric acid and dialysis steps used in the CEA-Roche assay, has been devised. Comparison of results on 746 plasma samples using our assay with those obtained using the CEA-Roche assay revealed a highly significant correlation coefficient (0.72; p < 0.00001). A further comparison of results on 373 plasma samples using our assay, but including a preincubation step with goat serum, with those obtained using the CEA-Roche assay also revealed a highly significant correlation coefficient (0.89; 0.00001). The goat serum preincubation step eliminated most of the discordant results in which our assay value was higher than CEA-Roche assay, presumably because of anti-goat antibodies in the patient plasma sample.
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