The human dynamin‐related protein OPA1 is anchored to the mitochondrial inner membrane facing the inter‐membrane space
2002; Wiley; Volume: 523; Issue: 1-3 Linguagem: Inglês
10.1016/s0014-5793(02)02985-x
ISSN1873-3468
AutoresAurélien Olichon, Laurent J. Emorine, Eric Descoins, Laetitia Pelloquin, Laetitia Brichese, Nicole Gas, Emmanuelle Guillou, Cécile Delettre, Annie Valette, Christian Hamel, Bernard Ducommun, Guy Lenaers, Pascale Belenguer,
Tópico(s)Photosynthetic Processes and Mechanisms
ResumoMutations in the OPA1 gene are associated with autosomal dominant optic atrophy. OPA1 encodes a dynamin‐related protein orthologous to Msp1 of Schizosaccharomyces pombe and Mgm1p of Saccharomyces cerevisiae , both involved in mitochondrial morphology and genome maintenance. We present immuno‐fluorescence and biochemical evidences showing that OPA1 resides in the mitochondria where it is imported through its highly basic amino‐terminal extension. Proteolysis experiments indicate that OPA1 is present in the inter‐membrane space and electron microscopy further localizes it close to the cristae. The strong association of OPA1 with membranes suggests its anchoring to the inner membrane.
Referência(s)