Artigo Produção Nacional Revisado por pares

Induced gene expression in oyster Crassostrea gigas exposed to sewage

2008; Elsevier BV; Volume: 26; Issue: 3 Linguagem: Inglês

10.1016/j.etap.2008.05.004

ISSN

1872-7077

Autores

Igor Dias Medeiros, Marília Nardelli Siebert, Guilherme Toledo‐Silva, T. B. Rodrigues, Maria Risoleta Freire Marques, Afonso Celso Dias Bainy,

Tópico(s)

Marine Bivalve and Aquaculture Studies

Resumo

Pacific oysters, Crassostrea gigas, were exposed to untreated sewage diluted in seawater. After 48h of exposure, the expression of genes associated to biotransformation pathways (CYP356A1, GSTO, MDR, FABP and ALAS) were analyzed in gills through semi-quantitative RT-PCR. A significant induction in all genes analyzed in the sewage-exposed oysters was observed. These genes are related to phase I (CYP356A1), phase II (GSTO) and phase III (MDR) biotransformation systems, to the uptake and transport of hydrophobic ligands (FABP) and to the synthesis of prosthetic group heme (ALAS). The organisms were able to survive in contaminated conditions since protective mechanisms have been properly stimulated.

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