
Induced gene expression in oyster Crassostrea gigas exposed to sewage
2008; Elsevier BV; Volume: 26; Issue: 3 Linguagem: Inglês
10.1016/j.etap.2008.05.004
ISSN1872-7077
AutoresIgor Dias Medeiros, Marília Nardelli Siebert, Guilherme Toledo‐Silva, T. B. Rodrigues, Maria Risoleta Freire Marques, Afonso Celso Dias Bainy,
Tópico(s)Marine Bivalve and Aquaculture Studies
ResumoPacific oysters, Crassostrea gigas, were exposed to untreated sewage diluted in seawater. After 48h of exposure, the expression of genes associated to biotransformation pathways (CYP356A1, GSTO, MDR, FABP and ALAS) were analyzed in gills through semi-quantitative RT-PCR. A significant induction in all genes analyzed in the sewage-exposed oysters was observed. These genes are related to phase I (CYP356A1), phase II (GSTO) and phase III (MDR) biotransformation systems, to the uptake and transport of hydrophobic ligands (FABP) and to the synthesis of prosthetic group heme (ALAS). The organisms were able to survive in contaminated conditions since protective mechanisms have been properly stimulated.
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