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Image-based adaptive optics for two-photon microscopy

2009; Optica Publishing Group; Volume: 34; Issue: 16 Linguagem: Inglês

10.1364/ol.34.002495

1539-4794

Delphine Débarre, Edward J. Botcherby, Tomoko Watanabe, Shankar Srinivas, Martin J. Booth, Tony Wilson,

Image Processing Techniques and Applications

We demonstrate wavefront sensorless aberration correction in a two-photon excited fluorescence microscope. Using analysis of the image-formation process, we have developed an optimized correction scheme permitting image-quality improvement with minimal additional exposure of the sample. We show that, as a result, our correction process induces little photobleaching and significantly improves the quality of images of biological samples. In particular, increased visibility of small structures is demonstrated. Finally, we illustrate the use of this technique on various fresh and fixed biological tissues.