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Targeted and Stable Gene Delivery into Muscle Cells by a Two-Step Transfer System

2000; Elsevier BV; Volume: 275; Issue: 3 Linguagem: Inglês

10.1006/bbrc.2000.3382

1090-2104

Isao Fujii, Satoru Suzuki, Takehito Igarashi, Makoto Matsukura, Teruhisa Miike, Takashi Shimada,

CAR-T cell therapy research

We developed a muscle-specific gene delivery system based on two-step gene transfer. The first step involved adenovirus-mediated transfer of the ecotropic retrovirus receptor (EcoRec) gene driven by the muscle-specific desmin promoter. Both human primary myoblasts and fibroblasts were efficiently transduced with this adenovirus vector. However, expression of EcoRec was detected only in myoblasts. In the second step, EcoRec-expressing myoblasts could be stably transduced with the ecotropic retroviral vector with the β-galactosidase gene. Approximately 15% of myoblasts were transduced by this two-step strategy. When the transduced myoblasts were differentiated into myotubes, extensive cell–cell fusion occurred, and the apparent number of β-galactosidase-positive cells increased to 28%. These results indicate that our two-step gene delivery system could be used for targeted and stable gene transfer into muscle cells.