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Flow cytometric differentiation of abnormal and normal plasma cells in the bone marrow in patients with multiple myeloma and its precursor diseases

2013; Elsevier BV; Volume: 38; Issue: 3 Linguagem: Inglês

10.1016/j.leukres.2013.12.007

1873-5835

Prashant Tembhare, Constance M. Yuan, David Venzon, Raul C. Braylan, Neha Korde, Elisabet E. Manasanch, Diamond Zuchlinsky, Katherine R. Calvo, Roger Kurlander, Manisha Bhutani, Nishant Tageja, Irina Marić, Marcia Mulquin, Mark Roschewski, Mary Kwok, David J. Liewehr, Ola Landgren, Maryalice Stetler‐Stevenson,

Peptidase Inhibition and Analysis

Flow cytometric (FC) enumeration of abnormal plasma cells (APCs) for diagnosis and prognostication of plasma cell dyscrasias (PCD) is challenging. We studied antigen expression in normal plasma cells (NPC) (N = 34) and APC in a series of unselected PCD (N = 59). NPC subpopulations often demonstrated CD19(-), CD20(+), CD45(-) or dim and CD56(+), an immunophenotype observed in PCD. However abnormal CD81 was only observed in APCs (APC detection sensitivity 95%; specificity 100%). We evaluated differences in antigen expression patterns among MGUS (N = 14), SMM (N = 35) and MM (N = 10), finding the combination of CD45 and CD56 helpful in differentiating MGUS from SMM and MM (p = 0.0002).