Live cell refractometry using microfluidic devices
2006; Optica Publishing Group; Volume: 31; Issue: 18 Linguagem: Inglês
10.1364/ol.31.002759
ISSN1539-4794
AutoresNiyom Lue, Gabriel Popescu, Takahiro Ikeda, Ramachandra R. Dasari, Kamran Badizadegan, Michael S. Feld,
Tópico(s)Advanced Fluorescence Microscopy Techniques
ResumoUsing Hilbert phase microscopy for extracting quantitative phase images, we measured the average refractive index associated with live cells in culture. To decouple the contributions to the phase signal from the cell refractive index and thickness, we confined the cells in microchannels. The results are confirmed by comparison with measurements of spherical cells in suspension.
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