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Purification and Identification of a Rhabdovirus from Thunbergia alata

1992; Wiley; Volume: 135; Issue: 2 Linguagem: Inglês

10.1111/j.1439-0434.1992.tb01257.x

1439-0434

R. G. Dietzgen, DH Gowanlock, John E. Thomas,

Bacteriophages and microbial interactions

Abstract A subgroup 2 rhabdovirus was isolated in south‐east Queensland from black‐eyed Susan ( Thunbergia alata ) with symptoms of vein yellowing, vein clearing and leaf distortion. Bacilliform particles accumulated in the perinuclear space of infected plants and measured 69 ± 7 × 161 ± 8 nm in unfixed preparations. The virus was not transmitted mechanically. Purified preparations of the Thunbergia alata rhabdovirus (TaRV) contained four major proteins with molecular weights of 80 kD, 48 kD, 40 kD and 35 kD, similar to those of datura yellow vein virus (DYW), a newly described rhabdovirus from Australia. The 80 kD protein was identified as the viral glycoprotein. In immunoblots, the two largest proteins of TaRV reacted strongly with antiserum to DYW, but were serologically distinct from sonchus yellow net, cereal chlorotic mottle, potato yellow dwarf and lettuce necrotic yellows viruses. TaRV is considered to be a strain of DYW.