Portal de Periódicos da CAPES

Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle

2009; SPIE; Volume: 14; Issue: 03 Linguagem: Inglês

10.1117/1.3130266

1560-2281

Silvia Santos, Kengyeh K. Chu, Daryl Lim, Nenad Bozinovic, Timothy N. Ford, Claire Hourtoule, Aaron C. Bartoo, Satish K. Singh, Jérôme Mertz,

Advanced Fluorescence Microscopy Techniques

We present an endomicroscope apparatus that exhibits out-of-focus background rejection based on wide-field illumination through a flexible imaging fiber bundle. Our technique, called HiLo microscopy, involves acquiring two images, one with grid-pattern illumination and another with standard uniform illumination. An evaluation of the image contrast with grid-pattern illumination provides an optically sectioned image with low resolution. This is complemented with high-resolution information from the uniform illumination image, leading to a full-resolution image that is optically sectioned. HiLo endomicroscope movies are presented of fluorescently labeled rat colonic mucosa.