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Measurement of Chlorophyll Fluorescence by Synchronous Detection in Integrating Sphere: A Modified Analytical Approach for the Accurate Determination of Photosynthesis Parameters for Whole Plants

1998; American Chemical Society; Volume: 32; Issue: 17 Linguagem: Inglês

10.1021/es971039f

1520-5851

Richard Salvetat, Philippe Juneau, Radovan Popovic,

Light effects on plants

In photosynthesis research, pulse-amplified modulated (PAM) fluorescence is used to study the chlorophyll fluorescence induction of plants in order to investigate the mechanisms of the energy transfer in photosynthesis and to determine the plant physiological state. However, in many cases the lack of accuracy and reproducibility in measurement introduces some problems in the determination of fluorescence parameters and therefore in their interpretation concerning environmental research domain. In this paper, a new type of modulated fluorometer designed to resolve these problems is presented. An integrating sphere was used as a measuring chamber associated with a PAM fluorometer system that was originally built in our laboratory. To demonstrate the performance and the reliability of our measuring system, we presented results of fluorescence measurements of plants that have been exposed to different environmental conditions (ultraviolet-B radiation, low- and high-temperature stress). The advantages of measuring different fluorescence parameters with synchronous detection by this type of PAM system are discussed.