Manipulation of cell cycle kinetics: Influence on the cytotoxicity of doxorubicin in human breast cancer cells
1990; Elsevier BV; Volume: 37; Issue: 6 Linguagem: Inglês
10.1016/0960-0760(90)90472-w
ISSN1879-1220
AutoresM. Bontenbal, Anieta M. Sieuwerts, Harm Peters, Pieter Sonneveld, J.A. Foekens, J.G.M. Klijn,
Tópico(s)Estrogen and related hormone effects
ResumoIn vitro exposure of estrogen receptor-negative (ER-) EVSA-T human breast cancer cells to insulin and/or estradiol had no effect on cell cycle distribution, in contrast to a 3–5-fold increase in the percentages of cells in the S-phase of the cell cycle in the ER+ MCF-7 cell line. Estrogen pretreatment of MCF-7 cells followed by incubation with doxorubicin resulted in an augmented inhibition of cell growth compared to unstimulated controls. This delay in growth was accompanied by a decrease in the percentages of cells actively synthesizing DNA, and by an augmented percentage of cells exhibiting a G2M-amount of DNA at the end of a 6–9 day period of culture in complete growth medium.
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