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Crystal structures of VAP1 reveal ADAMs' MDC domain architecture and its unique C-shaped scaffold

2006; Springer Nature; Volume: 25; Issue: 11 Linguagem: Inglês

10.1038/sj.emboj.7601131

1460-2075

Soichi Takeda, Tomoko Igarashi, Hidezo Mori, Satohiko Araki,

Cell Adhesion Molecules Research

Article11 May 2006free access Crystal structures of VAP1 reveal ADAMs' MDC domain architecture and its unique C-shaped scaffold Soichi Takeda Corresponding Author Soichi Takeda Department of Cardiac Physiology, National Cardiovascular Center Research Institute, Suita, Osaka, Japan Laboratory for Structural Biochemistry, Riken Harima Institute at SPring-8, Mikazuki, Sayo, Hyogo, Japan Search for more papers by this author Tomoko Igarashi Tomoko Igarashi Department of Cardiac Physiology, National Cardiovascular Center Research Institute, Suita, Osaka, Japan Search for more papers by this author Hidezo Mori Hidezo Mori Department of Cardiac Physiology, National Cardiovascular Center Research Institute, Suita, Osaka, Japan Search for more papers by this author Satohiko Araki Satohiko Araki Sugashima Marine Biological Laboratory, Graduate School of Science, Nagoya University, Toba, Mie, Japan Search for more papers by this author Soichi Takeda Corresponding Author Soichi Takeda Department of Cardiac Physiology, National Cardiovascular Center Research Institute, Suita, Osaka, Japan Laboratory for Structural Biochemistry, Riken Harima Institute at SPring-8, Mikazuki, Sayo, Hyogo, Japan Search for more papers by this author Tomoko Igarashi Tomoko Igarashi Department of Cardiac Physiology, National Cardiovascular Center Research Institute, Suita, Osaka, Japan Search for more papers by this author Hidezo Mori Hidezo Mori Department of Cardiac Physiology, National Cardiovascular Center Research Institute, Suita, Osaka, Japan Search for more papers by this author Satohiko Araki Satohiko Araki Sugashima Marine Biological Laboratory, Graduate School of Science, Nagoya University, Toba, Mie, Japan Search for more papers by this author Author Information Soichi Takeda 1,2, Tomoko Igarashi1, Hidezo Mori1 and Satohiko Araki3 1Department of Cardiac Physiology, National Cardiovascular Center Research Institute, Suita, Osaka, Japan 2Laboratory for Structural Biochemistry, Riken Harima Institute at SPring-8, Mikazuki, Sayo, Hyogo, Japan 3Sugashima Marine Biological Laboratory, Graduate School of Science, Nagoya University, Toba, Mie, Japan *Corresponding author. Department of Cardiac Physiology, National Cardiovascular Center Research Institute, Fujishirodai 5-7-1, Suita, Osaka, 565-8565, Japan. Tel.: +81 6 6833 5012 ext.2381; Fax: +81 6 6872 7485; E-mail: [email protected] The EMBO Journal (2006)25:2388-2396https://doi.org/10.1038/sj.emboj.7601131 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info ADAMs (a disintegrin and metalloproteinase) are sheddases possessing extracellular metalloproteinase/disintegrin/cysteine-rich (MDC) domains. ADAMs uniquely display both proteolytic and adhesive activities on the cell surface, however, most of their physiological targets and adhesion mechanisms remain unclear. Here for the first time, we reveal the ADAMs' MDC architecture and a potential target-binding site by solving crystal structures of VAP1, a snake venom homolog of mammalian ADAMs. The D-domain protrudes from the M-domain opposing the catalytic site and constituting a C-shaped arm with cores of Ca2+ ions. The disintegrin-loop, supposed to interact with integrins, is packed by the C-domain and inaccessible for protein binding. Instead, the hyper-variable region (HVR) in the C-domain, which has a novel fold stabilized by the strictly conserved disulfide bridges, constitutes a potential protein–protein adhesive interface. The HVR is located at the distal end of the arm and faces toward the catalytic site. The C-shaped structure implies interplay between the ADAMs' proteolytic and adhesive domains and suggests a molecular mechanism for ADAMs' target recognition for shedding. Introduction ADAMs (a disintegrin and metalloproteinase) or MDC (metalloproteinase/disintegrin/cysteine-rich) proteins comprise an emerging class of mammalian metalloproteinases with potential regulatory roles in cell–cell and cell–matrix adhesion and signalling (Becherer and Blobel, 2003; Seals and Courtneidge, 2003; White, 2003; Blobel, 2005). To date, over 30 ADAMs have been identified in a variety of species from fission yeast to human. Roughly, half of these are believed to function as active metalloproteinases and thus to constitute major membrane-bound sheddase that can proteolytically release cell-surface-protein ectodomains including growth factors and cytokines, their receptors and cell adhesion molecules. For example, ADAM17 (TACE, TNF-α converting enzyme) releases many cell-surface proteins including TNF-α precursor (Black et al, 1997; Moss et al, 1997) and ADAM10 (kuzbanian), which dictates lateral inhibition of Drosophila neurogenesis (Rooke et al, 1996), releases Notch ligand Delta (Qi et al, 1999) and Notch itself (Pan and Rubin, 1997). With regard to cellular interactions, fertilin α and β (ADAM1 and ADAM2, respectively) have been identified as sperm surface molecules essential for fertilization (Primakoff et al, 1987; Blobel et al, 1990, 1992) and meltrin α (ADAM12) is implicated in myogenesis (Yagami-Hiromasa et al, 1995). ADAMs have been associated with numerous diseases including arthritis, Alzheimer's disease, and cancer (Duffy et al, 2003; Moss and Bartsch, 2004). ADAM33 has been genetically linked with asthma (Van Eerdewegh et al, 2002). ADAMs uniquely display both proteolytic and adhesive activities on the cell surface, however, most of their physiological targets and the adhesion mechanisms remain unclear. Disintegrins are small proteins (40–90 aa) isolated from snake venom typically with an Arg-Gly-Asp (RGD) recognition sequence on an extended loop (disintegrin-loop) that inhibit platelet aggregation via integrin binding (Huang et al, 1987; Calvete et al, 2005). ADAMs are unique among cell surface proteins in possessing a disintegrin (D-) domain and thus it has been suggested that integrins might be common receptors for ADAMs (Blobel et al, 1992; Evans, 2001; White, 2003). However, the RGD sequence in the ADAMs' disintegrin-loop is usually replaced by XXCD and therefore, its adhesive potential has been controversial. Both the ADAMs' D- and cysteine-rich (C-) domains are involved in the protein–protein interactions (Myles et al, 1994; Almeida et al, 1995; Zolkiewska, 1999; Iba et al, 2000; Gaultier et al, 2002; Smith et al, 2002), however, the details of the interactions have remained elusive. This is because high-resolution structures have been available only for isolated domains (Maskos et al, 1998; Orth et al, 2004; Janes et al, 2005) and no structural information has been available for the C-domain of the canonical ADAMs. To clarify the molecular mechanisms of target recognition for shedding by and of cellular adhesion via ADAMs, elucidation of the atomic structure of the ADAMs' MDC domains is indispensable. To obtain structural data on an ADAM family member, we exploited the fact that hemorrhagic P-III snake venom metalloproteinases (SVMPs) share the ADAMs' MDC architecture (Jia et al, 1996; Evans, 2001; Fox and Serrano, 2005). Most ADAMs possess additionally, EGF-like, transmembrane and cytoplasmic domains and therefore are primarily membrane-associated, whereas SVMPs are secreted. Vascular apoptosis-inducing protein-1 (VAP1) is a disulfide-bridged homodimer P-III SVMP isolated from Crotalus atrox venom (Masuda et al, 1998, 2000). VAP1's stability and intrinsic two-fold symmetry enabled us to solve the crystal structures at 2.5-Å resolution. The structure reveals the residues that are important for stabilizing the MDC architecture are strictly conserved throughout the primary structure among all the known ADAMs. Therefore, the present structure represents the general architecture of ADAMs' MDC domains and provides insights into the molecular mechanism of the ADAMs' target recognition. Results Structure determination VAP1 yielded crystals readily, and initial phases were determined by molecular replacement method using the structure of P-I SVMP, acutolysin-C (1QUA) (Zhu et al, 1999) as a starting model. Although the initial model, with 99 identical residues out of 197, represented less than 50% of the total molecule, two distinct local noncrystallographic two-fold symmetry (NCS) operations (see below) allowed us to completely model the whole molecule. The native structures were determined from the crystals with two distinct space groups, P212121 and P41212, both at 2.5-Å resolution (Table I). Orthorhombic crystals were used for inhibitor soaking and the GM6001 ((3-(N-hydroxycarboxamido)-2-isobutyl-propanoyl-Trp-methylamide))-bound structure was determined at 3.0-Å resolution (Table I). In either crystal forms, the asymmetric unit contained one dimer molecule. The four monomers in the two crystal forms have almost identical structures, except for slight variations in their domain orientations, terminal residues, surface loops and active-site GM6001-binding region. Table 1. Data collection and refinement statistics Native (orthorhombic) Native (tetragonal) GM6001-bound Data collection Space group P212121 P41212 P212121 Cell dimensions a, b, c (Å) 86.7, 93.3, 137.7 93.9,93.9,244.8 86.3, 91.4, 136.0 α, β, γ (deg) 90, 90, 90 90, 90, 90 90, 90, 90 Resolution (Å) 50–2.50 (2.59–2.50) 50–2.50 (2.59–2.50) 50–2.95 (3.06–2.95) Rmergea 0.072 (0.369) 0.084 (0.380) 0.072 (0.367) I/σI 14.4 (2.9) 18.7 (7.1) 12.6 (4.3) Completeness (%) 99.4 (98.8) 99.7 (99.6) 99.9 (99.4) Redundancy 3.91 12.7 4.95 Refinement Resolution (Å) 50–2.50 (2.59–2.50) 50–2.50 (2.59–2.50) 50–2.95 (3.06–2.95) No. of reflections 38874 38786 23295 Rworkb/Rfreec 0.212/0.258 0.229/0.269 0.208/0.264 No. of atoms Protein 6558 6513 6558 Zn2+ 2 2 2 Ca2+ 4 4 4 Co3+ 1 1 N-acetyl glucosamine 56 42 56 GM6001 56 Water 205 165 35 B-factors Protein 44.9 51.2 55.4 Zn2+ 40.9 41.6 46.4 Ca2+ 43.5 52.4 49.3 Co3+ 35.5 56.8 N-acetyl glucosamine 69.8 65.1 75.8 GM6001 78.6 Water 39.8 41.5 37.0 R.m.s deviations Bond lengths (Å) 0.0052 0.0080 0.0038 Bond angles (deg) 1.18 1.39 0.92 a Rmerge=∑hkl∑I∣Ii(hkl)- ∣ / ∑hkl∑iIi(hkl), where Ii(hkl) is the ith intensity measurement of reflection hkl and 〈I(hlk)〉 is its average. b Rwork=∑(∣∣Fobs∣−∣Fcalc∣∣/∑∣Fobs∣. c Rfree=R-value for a randomly selected subset (5%) of the data that were not used for minimization of the crystallographic residual. Highest resolution shell is shown in parenthesis. For each data set, single crystal was used for measurement. MDC architecture The MDC architecture of VAP1 is shown in Figure 1A and B. The metalloproteinase (M-) domains in the dimer are related by NCS such that their active sites point in opposite directions and an intermolecular disulfide bridge is formed between symmetry-related Cys365 residues (Figure 1A). The M-domain is followed by a disintegrin (D-) domain that is further divided into Ds- and Da-domains (see below). The Ds-domain protrudes from the M-domain close to the Ca2+-binding site I (see below) opposing the catalytic site. The D-domain forms a C-shaped arm, together with the cysteine-rich (C-) domain, with its concave surface toward the M-domain. There are no direct interactions between the arm and the M-domain. Notably, the distal portion of the C-domain comes close to and faces toward the catalytic site in the M-domain. The C-terminus Tyr610 is located proximal to the boundary between the Da- and C-domains (Figure 1A and B). Aside from Cys365, each monomer contains 34 cysteinyl residues, all of which are involved in disulfide bonding, and their spacings are strictly conserved among ADAMs (Figure 2 and Supplementary Figure 1) except within the substrate-binding (between the helices H4 and H5) and the HVR (see below) regions. Figure 2 provides a selected subset of the sequence alignments and the entire alignments of VAP1 and 39 ADAM sequences, including all 23 human ADAMs so far available, can be found as Supplementary Figure 1. Figure 1.MDC architecture. (A) VAP1 dimer viewed from the NCS axis. The H0-helix, M-domain, linker, Ds-, Da-, Cw-, and Ch-domains and HVRs belonging to the one monomer are shown in red, yellow, gray, cyan, pink, gray, green and blue, respectively. The disulfide-linked counterpart is shown in gray. Zinc and calcium ions are represented as red and black spheres, respectively. The NAG (N-acetyl-glucosamine, in orange) moieties linked to Asn218, the calcium-mimetic Lys202 and the bound inhibitor GM6001 (GM, in green) are in ball-stick representations. (B) Stereo view of VAP1 monomer from the direction nearly perpendicular to (A). The helix numbers are labelled. (C) Superposition of the M-domains of ADAM33 (blue) and VAP1 (yellow). The calcium ion bound to site I and the zinc ion in ADAM33 are represented by black and red spheres, respectively. The disulfide bridges are indicated in black and blue letters for VAP1 and ADAM33, respectively. The QDHSK sequence for the dimer interface in VAP1 (residues 320–324) is in red. (D) Comparison of the calcium-binding site I structures of ADAM33 (blue) and VAP1 (yellow) in stereo. The residues in ADAM33 and in VAP1 are labelled in blue and black, respectively. A calcium ion and a water molecule bound to ADAM33 are represented as green and red spheres, respectively. The ammonium group of Lys202 in VAP1 occupies the position of the calcium ion in ADAM33. In ADAM33 (Orth et al, 2004), side-chain oxygen atoms of Glu213, Asp296 and Asn407, the carbonyl oxygen of Cys404 and a water molecule form the corners of a pentagonal bipyramid and ligand to the calcium ion. Download figure Download PowerPoint Figure 2.Sequence alignments of VAP1 and human ADAMs. The cysteinyl residues and the conserved residues are shaded in pink and yellow, respectively. Disulfide bridges, secondary structures and domains are drawn schematically. The HVR, calcium-binding site I, catalytic site and disintegrin-loop (D-loop) are boxed in blue, red, green and cyan, respectively. The hydrophobic ridges (H-ridges) are indicated. Calcium-binding sites II and III and the coordinating residues (shaded in red) are indicated. The NCBI accession numbers for the sequences are indicated. Download figure Download PowerPoint M-domain Each VAP1 M-domain corresponds to a very similar structure to that of ADAM33 (Orth et al, 2004), with a flat ellipsoidal shape having a central core made up of five stranded β-sheets and five α-helices and a conserved methionine (Met-turn) below the active site histidine residues, which bears the typical structural feature of metzincin family of metalloproteinases (Bode et al, 1993). However, they differ in the dimer interface and the loop structure around the substrate-binding site (Figure 1C) that corresponds to the variable region in the primary structure (between the helices H4 and H5, see Figure 2). The N-terminal helix (H0) is also unique in VAP1. The dimer interface is best characterized by the recognition sequence QDHSK (residues 320–324, see Figure 1C and Supplementary Figure 2A–C) and by Cys365, however these are not conserved among ADAMs; therefore, none of the ADAMs' M-domains are suggested to form a stable dimer as VAP1. A peptide-like hydroxamate inhibitor GM6001 binds to VAP1 (Figure 1A and B, and Supplementary Figure 2D and E) in exactly the same manner as in the marimastat-ADAM33 M-domain complex (Orth et al, 2004), suggesting that the catalytic sites of VAP1 and ADAM33 share a common substrate recognition mechanism. The ADAM33 M-domain structure suggests that most ADAMs have a Ca2+-binding site (designated Ca2+-binding site I) opposing the active-site cleft; however, in VAP1, the distal ammonium group of Lys202 substitutes for the Ca2+ ion (Figure 1D). Replacement of the calcium-coordinating glutamate residue with lysine also occurs in ADAM16, ADAM25 and ADAMs38–40 (Supplementary Figure 1). C-shaped arm The D-domain follows the M-domain, with a short linker that allows slightly variable domain orientations at V405 as a pivotal point (Figure 3C). The D-domain is further divided into two structural subdomains (Figure 3), the ‘shoulder’ (Ds-domain, residues 396–440) and the ‘arm’ (Da-domain, residues 441–487). The Ds- and Da-domains constitute a continuous C-shaped arm, together with the following N-terminus region of the C-domain which we designate the ‘wrist’ (Cw-domain, residues 488–505). There are three disulfide bonds in the Ds-domain, three in the Da-domain and one in the Cw-domain. The subdomains are connected by single disulfide bridges (Figures 2 and 3A) with slightly variable angles (Figure 3B). Figure 3.Arm structure. (A) Arm structure in stereo. The Ds-, Da-, and Cw-domains are in cyan, pink and light green, respectively. The calcium-coordinating residues and the disulfide bridges are shown in red and green, respectively. The residues with carbonyl oxygen atoms involved in calcium coordination are underlined. Calcium ions are represented as black spheres. The disintegrin-loop (DECD) is in blue. (B) Superimposition of the four Da-domains of VAP1 and trimestatin (1J2L). Trimestatin and its RGD loop are shown in red and blue, respectively. (C) Superimposition of the four Ds-domains. The linker between the M- and Ds-domains is shown in gray. Val405 at the pivotal point is indicated. Download figure Download PowerPoint Both the Ds- and Da-domains contain structural calcium-binding sites. In the Ds-domain, the side-chain oxygen atoms in residues Asn408, Glu412, Glu415 and Asp418, and the carbonyl oxygen atoms of Val405 and Phe410 are involved in pentagonal bipyramidal coordination and constitute Ca2+-binding site II (Figures 2 and 3A). Notably, these residues are strictly conserved among all known ADAMs (Supplementary Figure 1). However, the side-chain oxygens of Asp469, Asp472 and Asp483, and carbonyl oxygens of Met470 and Arg484 form the corners of a pentagonal bipyramid to the calcium ligand and constitute the Da-domain Ca2+-binding site III (Figures 2 and 3A) and these residues are highly conserved among ADAMs except ADAM10 and ADAM17 (Supplementary Figure 1). Because of the few secondary-structural elements, bound calcium ions and the disulfide bridges are essential for the structural rigidity of ADAM's C-shaped arm. The RGD-containing disintegrin trimestatin (Fujii et al, 2003) has a similar structure with the Da-domain (r.m.s.d of 1.24 Å, Figure 3B); however, no disintegrins have been shown to bind Ca2+ ions. Using isolated D-domains or portions thereof, numerous ADAMs and P-III SVMPs have been shown to interact specifically with particular integrins (Evans, 2001; White, 2003; Calvete et al, 2005). However, the disintegrin-loop is packed against the Cw-domain and a disulfide bridge (Cys468–Cys499) further stabilizes the continuous structure (Figure 3A). Therefore, the disintegrin-loop is inaccessible for protein binding. Hand domain The ‘hand’ domain (Ch-domain, residues 505–610) follows the Cw-domain. The Ch-domain, together with the Cw-domain, constitutes a novel fold (Figure 4A). In either crystal form, VAP1 dimers interact with molecules of neighboring units through the Ch-domains such that the molecules form a handshake (Figure 4B). Each Ch-domain interacts with its counterpart through a relatively large complementary surface of 860 Å2 forming another NCS at the center, although VAP1 exists as dimers, not as oligomers, and is mono-dispersed in solution (data not shown). Figure 4.C-domain architecture and HVR. (A) The C-domain architecture in stereo. The Cw- and Ch-domains are in gray and light green, respectively. The disulfide bridges and the residues forming the hydrophobic ridges are indicated. The HVR and its NCS counterpart are shown in red and blue, respectively. The variable loop (residues 539–549), flanked by two adjacent cysteine residues, is in green. (B) Crystal packing in the orthorhombic crystal. The crystallographically equivalent molecules (HVRs) are in cyan (blue) and pink (red), respectively. The arrows indicate the directions of the HVR chains. Zinc and calcium ions are represented as red and black spheres, respectively. (C) Interactions between the HVRs (cyan and pink) in stereo. The molecular surface of the cyan molecule is shown with the electrochemical surface potential (red to blue). The residues constituting the hydrophobic ridges are in yellow. The residues are labelled in blue and red for cyan and pink, respectively. (D) Water-mediated hydrogen-bond network in the HVR. The HVR residues are in pink and cyan; non-HVR residues in the pink molecule are in gray. Download figure Download PowerPoint HVR as a potential adhesive interface Ch-domain residues 562–583 are predominantly involved in the handshake (Figure 4B). This is the region in which the ADAM sequences are most divergent and variable in length (16–55 aa) (Figure 2 and Supplementary Figure 1). We have designated this as the hyper-variable region (HVR). The HVR is subdivided into two structural elements. The N-terminal portion (residues 562–572) fits into an extended loop, filling the gap between the M-domain and the neighboring molecule's Ch-domain and thus fixing the position of the arm (Figure 4B). The variable structures and less-specific interactions suggest that this loop is stabilized by crystal packing. Some ADAMs possess a putative fusion peptide in this segment typical of viral fusion proteins (Blobel et al, 1992; Yagami-Hiromasa et al, 1995), although their role in the actual fusion process has not been demonstrated. However, the remainder of the HVR (residues 572–583) interacts extensively with its counterpart by forming an antiparallel β strand at the center (Figure 4C and D). Although the ability to form β strand is predictable from the sequence, this β strand is stabilized mainly by interchain interactions (Figure 4D). There are no intrachain hydrogen bonds between residues 574–577 and the remainder of the Ch-domain; however a water-mediated hydrogen-bond network stabilizes this segment (Figure 4D). Therefore, it appears, that this β strand might be formed by the induced-fit mechanism upon the association of the Ch-domains and that the conserved disulfide bond (Cys526–Cys572, see Figure 4D) may stabilize the structure when the HVRs are isolated in solution. In addition to the main-chain hydrogen bonds, side-chain atoms (particularly residues I574, Y575, Y576 and P578) in the HVR β strand contribute numerous von der Waals interactions with their counterparts. Aside from the HVR, aromatic residues located at both sides of the β strand in close proximity to the NCS axis create additional interaction surfaces: residues Phe515, Gly516, His535 and Tyr536 in the loop regions form hydrophobic ridges that fit complementarily into the NCS region (Figure 4C). The hydrophobic ridges are highly conserved among ADAMs (Figure 2 and Supplementary Figure 1), thus, in part, they may also constitute binding surfaces. Discussion The VAP1 structures reveal highly conserved structural calcium-binding sites and the numbers and the spacings of cysteinyl residues that are essential for maintaining structural rigidity and spatial arrangement of the ADAMs' MDC domains. The C-shaped MDC architecture implies meaningful interplay between the domains and their potential roles in physiological functions. The HVR creates a novel interaction interface in collaboration with the conserved hydrophobic ridges. Different ADAMs have distinct HVR sequences, which result in distinct surface features, thus, they may function in specifying binding proteins. The HVR is at the distal end of the C-shaped arm and points toward the M-domain catalytic site, with a distance of ∼4 nm in between them. Collectively, these observations suggest that the HVR captures the target or associated protein that is processed by the catalytic site (Figure 5). The disintegrin portion is located opposit to and apart from the catalytic site and, thus, might play a primary role as a scaffold that allocates these two functional units spatially. The C-shaped structure also implies how the ADAMs' C-domains cooperate with their M-domains (Reddy et al, 2000; Smith et al, 2002). In membrane-bound ADAMs, the EGF-like domain (∼60 aa) follows the Ch-domain (Figure 2) and presumably works as a rigid spacer connecting the MDC-domains with and orientating against the membrane-spanning region (Figure 5A). Many ADAMs are proteolytically inactive (because of the defects in the catalytic HEXXHXXGXXHD sequence or the post-translational removal of the M-domain), and several of these are important developmentally. Therefore, the HVR may also work to modulate cell–cell and cell–matrix interactions. There is some experimental evidence for C-domain-mediated adhesion. Peptides encompassing the HVR and the hydrophobic ridge from P-III SVMPs interfere with platelet interaction and collagen binding (Kamiguti et al, 2003). A recombinant atrolysin-A C-domain specifically binds collagen I and von Willebrand factor (vWF) and blocks collagen–vWF interaction (Jia et al, 2000; Serrano et al, 2005). ADAM12 interacts with cell-surface syndecan through its C-domain and mediates integrin-dependent cell spreading (Iba et al, 2000). The D/C-domain portion of ADAM13 binds to the ECM proteins laminin and fibronectin (Gaultier et al, 2002). However, most of these studies do not assign specific regions of the C-domain to these interactions and the molecular recognition mechanisms are to be elucidated. Figure 5.Models for ADAM's shedding. The molecular surface of the VAP1 monomer, without VAP1's unique H0-helix, are colored as in Figure 1A. Hydrophobic ridges are in yellow. EGF-like, transmembrane and cytoplasmic domains are represented schematically. (A) Membrane-anchored substrate molecule ‘X’ is directly recognized and captured by the HVR on the membrane-bound ADAM molecule. The distance between the center of the HVR (Tyr575) and the catalytic zinc ion is about 3.5 nm. (B) Substrate ‘X’ is recognized by the ADAM HVR via binding with an associated protein ‘Y’. (C) ADAM cleaves substrate ‘X’ in trans via binding with an associated protein ‘Y’. Download figure Download PowerPoint ADAM10 and ADAM17 lack the Ca2+-binding site III and show less sequence similarities in the C-domain with other canonical ADAMs (Supplementary Figure 1). Comparison of the recently solved ADAM10 D/C-domain partial structure (ADAM10D+C) (Janes et al, 2005) and that of VAP1 reveals that the atypical ADAM10 shares the continuous Da/Cw structure and the Ch-domain scaffold with VAP1; however, it has an disordered Ds-domain and an alternate HVR structure and a different orientation between Cw- and Ch-domains (Figure 6). The locations of four of the five disulfide bridges within the Ch-domain are conserved between VAP1 and ADAM10 (Figure 6B and C) and thus, they enabled us to align the two sequences (Figure 6E). Based on this alignment, we completed entire alignments (Supplementary Figure 1) including 38 sequences of mammalian ADAMs and Schizosaccharomyces pombe Mde10 (Nakamura et al, 2004), presumably the founding member of the ADAM family in evolutionary terms. The ADAM10D+C structure lacks the eight residues (583–590 in ADAM10) that may form a flexible loop. However, VAP1 (Figure 6E) and the canonical ADAMs except for ADAM8 (Supplementary Figure 1) have extra 16 residues in this segment that, in part, forms a variable loop, flanked by the adjacent cysteinyl residues (Cys539 and Cys549 in VAP1) and protrudes from the main body of the C-domain (Figures 4A and 6B). The variable loop has highest temperature factor in the molecule and resembles to the disintegrin-loop, thus can be an additional protein-binding interface. The six VAP1 monomer molecules represent almost the same Cw/Ch domain orientation (data not shown), however that is distinct from that of ADAM10 (Figure 5A). Thus, the possibility whether different ADAMs have distinct Cw/Ch domain orientation remains to be established. Janes et al (2005) have shown that the three glutamate residues outside of HVR are essential for ADAM10-mediated ephrin proteolysis in trans, however, roles of the ADAM10 HVR has not been examined. An extensive molecular surface of the elongated arm structure (12 000 Å2 for the VAP1 D/C-domains) might reveal additional protein–protein interaction interfaces other than the HVR. Multiple charged residues in the D-domain are essential for ADAM28 binding to α4β1 (Bridges et al, 2003) and the RX6DLPEF motif has been proposed for integrin α9β1 binding (Eto et al, 2002). However, the D-domain portion of the C-shaped scaffold is away from the catalytic site; thus, those additional sites might not directly serve as target recognition interfaces for catalysis. Figure 6.Comparison of the VAP1 and ADAM10 D/C domains. (A) Superimposition of the Da-domains of ADAM10 and VAP1. The Ds/Da/Cw-domains and the H7 helix of VAP1 and those of ADAM10 are shown in blue and red, respectively. The Ch-domains of VAP1 and ADAM10 are shown in cyan and pink, respectively. The arrow indicates the pivotal point between the Cw- and Ch-domains. Bound Ca2+ ions in VAP1 are shown as black spheres. (B) Ribbon representation of the Ch-domain of VAP1. The HVR is shown in blue. The common scaffold between the VAP1 and ADAM10 Ch-domains are shown in cyan and the segment lacking in ADAM10 is shown in light green. Disulfide bridges are indicated. (C) Ribbon representation of the Ch-domain of ADAM10. The HVR is shown in red. Disulfide brides are indicated. (D) Superimposition of the Ch-