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Antibiotic Produced by Fusarium equiseti NRRL 5537

1974; American Society for Microbiology; Volume: 5; Issue: 6 Linguagem: Inglês

10.1128/aac.5.6.634

1098-6596

H. R. Burmeister, G. A. Bennett, R. F. Vesonder, C. W. Hesseltine,

Essential Oils and Antimicrobial Activity

Fusarium equiseti NRRL 5537 grown on an autoclaved white corn grit medium for 3 to 4 weeks at room temperature produced a substance in excess of 5 g/kg of substrate that inhibited some gram-positive bacteria including mycobacteria. Most Bacillus subtilis, Mycobacterium phlei , and Staphylococcus aureus strains were inhibited when 1 μg of the antibiotic per ml was incorporated into the culture medium. Except for Neisseria perflava , gram-negative bacteria, yeasts, and molds were not inhibited by 128 μg/ml. The antibiotic was recovered as a white powder, had a melting point of 65 to 66 C, and had an intraperitoneal mean lethal dose in white mice of 63 mg/kg of body weight. In thin-layer chromatographic analysis the compound appeared as a single spot in two different solvent systems. Mass spectrometry determined that the molecular weight of the antibiotic was 373 with a molecular formula of C 22 H 31 NO 4 . Chemical microanalysis was in accord with the formula.