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Colorimetric Detection of PCR Product with DNAzymes Induced by 5′‐Nuclease Activity of DNA Polymerases

2010; Wiley; Volume: 12; Issue: 1 Linguagem: Inglês

10.1002/cbic.201000650

1439-7633

Feng Du, Zhuo Tang,

Aquaculture disease management and microbiota

ChemBioChemVolume 12, Issue 1 p. 43-46 Communication Colorimetric Detection of PCR Product with DNAzymes Induced by 5′-Nuclease Activity of DNA Polymerases Feng Du, Feng Du Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041 (China), Fax: (+86) 28-85243250Search for more papers by this authorDr. Zhuo Tang, Corresponding Author Dr. Zhuo Tang tangzhuo@cib.ac.cn Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041 (China), Fax: (+86) 28-85243250Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041 (China), Fax: (+86) 28-85243250Search for more papers by this author Feng Du, Feng Du Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041 (China), Fax: (+86) 28-85243250Search for more papers by this authorDr. Zhuo Tang, Corresponding Author Dr. Zhuo Tang tangzhuo@cib.ac.cn Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041 (China), Fax: (+86) 28-85243250Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041 (China), Fax: (+86) 28-85243250Search for more papers by this author First published: 15 December 2010 https://doi.org/10.1002/cbic.201000650Citations: 16Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Detection made easy: A colorimetric probe for the sequence-specific detection of PCR reactions was designed to be partially complimentary to the amplified DNA target. Hybridization leads to DNA polymerase cleavage of the probe to release a peroxidase-like DNAzyme fragment, which binds with hemin to oxidize ABTS into a green product with H2O2. Citing Literature Supporting Information Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Filename Description cbic_201000650_sm_miscellaneous_information.pdf394.4 KB miscellaneous_information Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume12, Issue1January 3, 2011Pages 43-46 RelatedInformation