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In vivo synthesis of lipids in rabbit iris, cornea and lens tissues

1970; Elsevier BV; Volume: 9; Issue: 1 Linguagem: Inglês

10.1016/s0014-4835(70)80063-x

1096-0007

T.W. Culp, Richard D. Cunningham, Philip W. Tucker, J. Jeter, L.H. Detterman,

Drug Transport and Resistance Mechanisms

In vivo experiments were performed to establish whether lipid-synthesizing pathways exist in iris, cornea and lens tissues. [1-14C]sodium acetate administered into the anterior chamber of each eye of young rabbits was used as a metabolic precursor. Four hours after introduction of the isotope, total lipids were extracted and characterized using gas-liquid and thin-layer chromatographic techniques. Radio-activity of the lipids was determined and data reported as (1) mumoles of [14C]acetate incorporated per tissue into fatty acids and cholesterol, and (2) % of [14C]acetate incorporated into individual complex lipids. A higher incorporation was observed for fatty acids suggesting a more rapid synthesis of these compounds from labeled acetate. Data are also presented which demonstrate the capacity of these tissues to incorporate labeled fatty acids into lecithin, cephalin and other complex lipids.