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Protein identification using 2D-LC-MS/MS

2005; Elsevier BV; Volume: 35; Issue: 3 Linguagem: Inglês

10.1016/j.ymeth.2004.08.016

1095-9130

Claire Delahunty, John R. Yates,

Metabolomics and Mass Spectrometry Studies

Multidimensional liquid chromatography techniques have been coupled to tandem mass spectrometry to provide a robust method to identify proteins in complex mixtures. Data acquisition is interfaced directly with search algorithms for identification through cross-correlation with databases. This review describes the most recent advances in methodologies for protein identification by mass spectrometry and describes the limitations of the application of the technologies.