Inhibition of bovine α‐glucosidase by Castanospermum australe and its effect on the biochemical identification of heterozygotes for generalised glycogenosis type II (Pompe's disease) in cattle
1987; Wiley; Volume: 64; Issue: 9 Linguagem: Inglês
10.1111/j.1751-0813.1987.tb15956.x
ISSN1751-0813
AutoresK. G. Reichmann, JO TWIST, R. A. McKenzie, K.J. Rowan,
Tópico(s)Neurological diseases and metabolism
ResumoSUMMARY: All 18 2‐year‐old Brahman bulls grazing in a paddock containing Castanospermum australe trees were diagnosed as heterozygotes for Pompe's disease by measurement of mononuclear cell a‐glucosidase activity. However, removal of the bulls to a paddock free of C. australe and retesting 2 months later indicated that 15 were homozygous normal. An in vitro assay demonstrated that a crude aqueous extract of seeds from these C. australe trees contained a potent inhibitor of mononuclear cell a‐glucosidase. Two Hereford steers were dosed with 0.6 g C. australe seed/kg bodyweight for 6 days. The α–glucosidase activity in blood mononuclear cells declined to 5% of normal within 48 h of commencement of dosing. It was therefore assumed that the bulls had consumed C. australe seeds. A means of differentiating true heterozygotes from animals consuming the toxic seed, using the ratio of plasma α‐glucosidase activity at pH 5.6 to that at pH 3.7, is proposed. Aust Vet J 64: 274–276
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