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Detection of <i>Nesopora caninum</i>-Specific DNA from Cerebrospinal Fluid by Polymerase Chain Reaction in a Dog with Confirmed Neosporosis

2012; Japanese Society of Veterinary Science; Volume: 74; Issue: 8 Linguagem: Inglês

10.1292/jvms.11-0341

1347-7439

Kyohei ISHIGAKI, Masahiko Noya, Yumiko Kagawa, Kazunori Ike, Hiromitsu Orima, Soichi IMAI,

Coccidia and coccidiosis research

A one-month male Greyhound dog presented with a swinging gait of the hindlimbs, and later developed muscular atrophy of the femoral region and hyperextension of hindlimbs. The dog had positive serum IFAT titers to Neospora caninum, but a negative titer in the cerebrospinal fluid (CSF). N. caninum-specific DNA was amplified from the CSF using a semi-nested polymerase chain reaction assay. Clusters of protozoa in biopsied muscle fibers were subsequently confirmed as N. caninum tachyzoites by immunohistochemical examination. Early recognition and treatment are necessary for effective recovery of clinical canine neosporosis, but antemortem diagnosis is difficult. We suggest that the detection of parasite deoxyribonucleic acid in the CSF is a useful antemortem diagnostic method in facilitating treatment of this disease.