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Radiochemical assay of glutathione S-epoxide transferase and its enhancement by phenobarbital in rat liver in vivo

1975; Elsevier BV; Volume: 24; Issue: 17 Linguagem: Inglês

10.1016/0006-2952(75)90080-5

1873-2968

Jukka Marniemi, Max G. Parkki,

Genomics, phytochemicals, and oxidative stress

A rapid and sensitive assay of glutathione S-epoxide transferase was developed using tritium-labeled styrene oxide as the epoxide substrate. The method is based on the separation of unreacted styrene oxide from the glutathione conjugate formed after the incubation by the extraction with light petroleum. The radioactivity of the conjugate remaining in the aqueous phase can be determined subsequently by liquid scintillation. Using the present radiochemical method we found, after the intraperitoneal administration of phenobarbital (80 mg/kg) in vivo, a significant enhancement (about 56%) in the conjugation of styrene oxide with glutathione catalyzed by rat liver soluble fraction. No enhancement in the enzyme activity could be detected, on the other hand, after administration with styrene (1 g/kg) or 20-methylcholanthrene (20 mg/kg).