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Characterization and performance of a neutral hydrophilic coating for the capillary electrophoretic separation of biopolymers

1993; Elsevier BV; Volume: 652; Issue: 1 Linguagem: Inglês

10.1016/0021-9673(93)80655-r

1873-3778

Dieter Schmalzing, Christine Piggee, František Foret, Emanuel Carrilho, Barry L. Karger,

Microfluidic and Bio-sensing Technologies

Polyvinylmethylsiloxanedol (50% vinyl) was synthesized and combined with a cross-linker for static coating onto fused-silica columns. After cross-linking and binding to the surface, linear polyacrylamide was grafted to the double bonds of the siloxanediol; subsequently, this linear polymer matrix was cross-linked with formaldehyde. The grafted neutral polymeric layer provided suppression of electroosmotic flow and minimized adsorption. This combination yielded successful open tube and polymer network separations of proteins, peptides and DNA molecules. Very high efficiencies (ca. 1 · 106 plates/m) were achieved for open tube protein separations, and hundreds of consecutive runs were performed with minimal change in migration times.